Plant Molecular Biology

, Volume 77, Issue 1–2, pp 17–31 | Cite as

A cotton group C MAP kinase gene, GhMPK2, positively regulates salt and drought tolerance in tobacco

  • Liang Zhang
  • Dongmei Xi
  • Shanwei Li
  • Zheng Gao
  • Shuoli Zhao
  • Jing Shi
  • Changai Wu
  • Xingqi Guo
Article

Abstract

Mitogen-activated protein kinase (MAPK) cascades play important roles in mediating biotic and abiotic stress responses. In plants, MAPKs are classified into four major groups (A–D) according to their sequence homology and conserved phosphorylation motifs. Compared with well-studied MAPKs in groups A and B, little is known about group C. In this study, we functionally characterised a stress-responsive group C MAPK gene (GhMPK2) from cotton (Gossypium hirsutum). Northern blot analysis indicated that GhMPK2 was induced by abscisic acid (ABA) and abiotic stresses, such as NaCl, PEG, and dehydration. Subcellular localization analysis suggested that GhMPK2 may activate its specific targets in the nucleus. Constitutive overexpression of GhMPK2 in tobacco (Nicotiana tabacum) conferred reduced sensitivity to ABA during both seed germination and vegetative growth. Interestingly, transgenic plants had a decreased rate of water loss and exhibited enhanced drought and salt tolerance. Additionally, transgenic plants showed improved osmotic adjustment capacity, elevated proline accumulation and up-regulated expression of several stress-related genes, including DIN1, Osmotin and NtLEA5. β-glucuronidase (GUS) expression driven by the GhMPK2 promoter was clearly enhanced by treatment with NaCl, PEG, and ABA. These results strongly suggest that GhMPK2 positively regulates salt and drought tolerance in transgenic plants.

Keywords

Cotton (GossypiumhirsutumGhMPK2 Salt Drought Osmotic adjustment Transgenic tobacco 

Abbreviations

ABA

Abscisic acid

GFP

Green fluorescence protein

GUS

β-Glucuronidase

IPCR

Inverse polymerase chain reaction

MAPK

Mitogen-activated protein kinase

OA

Osmotic adjustment

ORF

Open reading frame

PCR

Polymerase chain reaction

PEG

Polyethylene glycol

Notes

Acknowledgments

This work was financially supported by China National Transgenic Plant Research and Commercialization Projects (2009ZX08009-092B; 2009ZX08009-113B), and the National Natural Science Foundation of China (30970225).

Supplementary material

11103_2011_9788_MOESM1_ESM.tif (549 kb)
Supplementary material 1 (TIFF 497 kb)
11103_2011_9788_MOESM2_ESM.doc (32 kb)
Supplementary material 2 (DOC 33 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Liang Zhang
    • 1
  • Dongmei Xi
    • 2
  • Shanwei Li
    • 1
  • Zheng Gao
    • 1
  • Shuoli Zhao
    • 3
  • Jing Shi
    • 1
  • Changai Wu
    • 1
  • Xingqi Guo
    • 1
  1. 1.State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop BiologyShandong Agricultural UniversityTaianPeople’s Republic of China
  2. 2.Experimental CenterLinyi UniversityLinyiPeople’s Republic of China
  3. 3.College of Food Science and EngineeringShandong Agricultural UniversityTaianPeople’s Republic of China

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