The soybean GmbZIP1 transcription factor enhances multiple abiotic stress tolerances in transgenic plants
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Abscisic acid (ABA)-responsive element binding proteins (AREBs) are basic domain/leucine zipper transcription factors that bind to the ABA-responsive element (ABRE) in the promoter regions of ABA-inducible genes in plants. A novel bZIP transcription factor gene, GmbZIP1, encoding 438 amino acids with a conserved bZIP domain composed of 60 amino acids was isolated from salt-tolerant soybean cv. Tiefeng 8. Southern blotting showed that only one copy was present in the soybean genome. Phylogenetic analyses showed that GmbZIP1 belonged to the AREB subfamily of the bZIP family and was most closely related to AtABF2 and OsTRAB1. The expression of GmbZIP1 was highly induced by ABA, drought, high salt and low temperature; and GmbZIP1 was expressed in soybean roots, stems and leaves under different stress conditions. GmbZIP1 was localized inside the nuclei of transformed onion epidermal cells. Overexpression of GmbZIP1 enhanced the responses of transgenic plants to ABA and triggered stomatal closure under stresses, potentially leading to improved tolerances to several abiotic stresses such as high salt, low temperature and drought in transgenic plants. Furthermore, overexpression of GmbZIP1 affected the expression of some ABA or stress-related genes involved in regulating stomatal closure in Arabidopsis under ABA, drought and high salt stress conditions. A few AREB elements were detected in the promoter region of those ABA or stress-related genes, suggesting that GmbZIP1 regulates the ABA response or stomatal closure mediated by those downstream genes in transgenic Arabidopsis. Moreover, GmbZIP1 was used to improve the drought tolerance trait of Chinese wheat varieties BS93. Functional analysis showed that overexpression of GmbZIP1 enhanced the drought tolerance of transgenic wheat, and transcripts of GmbZIP1 were detected in transgenic wheat using RT-PCR. In addition, GmbZIP1 overexpression did not result in growth retardation in all transgenic plants, suggesting that GmbZIP1 may be a valuable genetic resource for engineering stress tolerance of crops.
KeywordsSoybean bZIP transcription factor ABA Abiotic stress tolerance Transgenic wheat
We are thankful to the reviewers for their comments and suggestions. We are also grateful to Dr. Robert McIntosh (University of Sydney), Dr. Mao Long (Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture in Institute of Crop Science, CAAS) and International Science Editing Ltd for modifying this manuscript. The work was funded in part by the National Key Project for Research on Transgenic Biology (2008ZX08002-002), the National 863 High-tech Project (2008AA10Z124), the National Natural Science Foundation of China (30700504 and 30700508), the Beijing Nova Program (2008B035), Natural Science Foundation of Beijing (5102016) and the Young Science Foundation of Beijing Academy of Agricultural and Forestry Science.
- Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez Mohammad M, Seki M, Keiichiro H, Masaru OT, Shinozaki K, Yamaguchi-Shinozaki K (2005) AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell 17:3470–3488PubMedCrossRefGoogle Scholar
- Jin XF, Xiong AS, Peng RH, Liu JG, Gao F, Chen JM, Yao QH (2010) OsAREB1, an ABRE-binding protein responding to ABA and glucose, has multiple functions in Arabidopsis. Biochem Mol Biol Rep 43(1):34–39Google Scholar
- Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K (2000) Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc Natl Acad Sci USA 97:11632–11637PubMedCrossRefGoogle Scholar
- Weltmeier F, Rahmani F, Ehlert A, Dietrich K, Wang X et al (2009) Expression patterns within the Arabidopsis C/S1 bZIP transcription factor network: availability of heterodimerization partners controls gene expression during stress response and development. Plant Mol Biol 69:107–119PubMedCrossRefGoogle Scholar
- Xiong L, Schumaker K, Zhu JK (2002) Cell signaling during cold, drought and salt stress. Plant Cell 14(suppl):165–183Google Scholar
- Yoshida T, Fujita Y, Sayama H, Kidokoro S, Maruyama K, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K (2010) AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J 61(4):672–685PubMedCrossRefGoogle Scholar
- Zheng HJ, He SJ (1994) Some improvements of the biolistic transformation system for Oryza sativa L. Chinese Biotechnol 12:111–115Google Scholar