Plant Molecular Biology

, Volume 74, Issue 6, pp 605–615 | Cite as

Molecular cloning and functional characterization of OsJAG gene based on a complete-deletion mutant in rice (Oryza sativa L.)

  • Yuanlin Duan
  • Zhijuan Diao
  • Huaqing Liu
  • Meishuang Cai
  • Feng Wang
  • Tao Lan
  • Weiren WuEmail author


In this article, we report an independent work of positional cloning and functional characterization of OsJAG gene in rice. The merit of our work is that we used a genuine null mutant, in which the wild-type allele was completely deleted. This allowed us to identify the mutant phenotypes accurately without the interference of residual function of the target gene. OsJAG is an important gene with pleiotropy, expressing almost throughout the plant and acting in both vegetative phase and reproductive phase. But its main and crucial roles are in regulating the development of all floral organs, especially in specifying the identity of stamens. Interestingly, OsJAG does not affect the number of floral organ primordial and so of floral organs in each whorl, suggesting that OsJAG does not influence the initiation of floral organ primordia, but affect the developmental fate of all floral organs after their primordia have initiated. Loss of OsJAG function results in maldevelopment of all floral organs, such as degenerated lemma and palea, elongated lodicules and deformed and sterile pistil. The stamen appears to be more sensitive to the mutation. All the six stamens in a mutant floret were thoroughly transformed into six pistil-like organs developed at the presumptive positions of the stamens in whorl 3.


Oryza sativa OsJAG Floral organ development Stamen specification 







Oryza sativa JAGGED


Polymerase chain reaction


Scanning electron microscopy




Semi-quantitative reverse transcription PCR


Simple sequence repeat



This work was supported by Natural Science Foundation of Fujian Province (Grant no.: B0620001), National Hi-Tech Research and Development Program of China (Grant no.: 2006AA10Z128), National Natural Science Foundation of China (Grant no.: 30671122), National Key Sci-Tech Project of Breeding New Transgenic Varieties (Grant no.: 2009ZX08009-109B), and Fujian University New Century Talents Supporting Program (Grant no.: 0608566). We thank Prof. Yuanchang Zhou, Dr. Zhiwei Chen, Assoc. Prof. Runseng Pan, Assoc. Prof. Lihui Lin, Mr. Huazhong Guan and Mr. Xuzhang Zhang for their help in the experiment.

Supplementary material

11103_2010_9703_MOESM1_ESM.doc (27 kb)
Supplementary material 1 (DOC 27 kb)
11103_2010_9703_MOESM2_ESM.doc (25 kb)
Supplementary material 2 (DOC 25 kb)


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Yuanlin Duan
    • 1
    • 2
  • Zhijuan Diao
    • 1
    • 3
  • Huaqing Liu
    • 4
  • Meishuang Cai
    • 1
    • 2
  • Feng Wang
    • 4
  • Tao Lan
    • 1
    • 2
  • Weiren Wu
    • 1
    • 2
    Email author
  1. 1.Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of CropsFujian Agriculture and Forestry UniversityFuzhouPeople’s Republic of China
  2. 2.Fujian Provincial Key Laboratory of Marker-Assisted Breeding of RiceFujian Agriculture and Forestry UniversityFuzhouPeople’s Republic of China
  3. 3.Key Lab of Genetic Improvement for SugarcaneMinistry of AgricultureFuzhouPeople’s Republic of China
  4. 4.Fujian Academy of Agricultural SciencesFuzhouPeople’s Republic of China

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