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Plant Molecular Biology

, Volume 74, Issue 4–5, pp 467–478 | Cite as

A RING finger E3 ligase gene, Oryza sativa Delayed Seed Germination 1 (OsDSG1), controls seed germination and stress responses in rice

  • Gi-Gyeong Park
  • Jong-Jin Park
  • Jinmi Yoon
  • Sun-Nam Yu
  • Gynheung AnEmail author
Article

Abstract

Seed germination is an important character for plant growth and seed quality. We identified a rice mutant that was delayed in its germination. There, T-DNA was inserted into Oryza sativa Delayed Seed Germination 1 (OsDSG1), causing a recessive null mutation. Overexpression of the gene enhanced seed germination. OsDSG1 is most similar to Arabidopsis AIP2, an E3 ligase targeting ABI3.Yeast two-hybrid experiments showed that our OsDSG1 binds to OsABI3, indicating that OsDSG1 is a rice ortholog of AIP2. Self-ubiquitination assay indicated that bacterially expressed OsDSG1 protein has E3 ubiquitin ligase activity. Real-time PCR analysis revealed that OsDSG1 was expressed in leaves and roots, and strongly in developing seeds. In addition to the delayed-germination phenotype, mutant plants were shorter and had greater tolerance to high-salt and drought stresses. In the osdsg1 mutant, transcript levels of ABA signaling genes and ABA responsive genes were significantly increased. By contrast, expressions of OsGAMYB and its downstream genes that encode hydrolytic enzymes were markedly reduced. These observations support that OsDSG1 is a major regulator of ABA signaling in germinating seeds. Finally, we observed that the germination rates of various rice cultivars depended upon the transcript levels of OsDSG1 and other ABA-signaling genes.

Keywords

ABA signaling Abiotic stresses E3 ligase Rice Seed germination 

Notes

Acknowledgments

We thank Kyungsook An for plant transformation, Priscilla Licht for English editing, Gihwan Yi for providing the rice seeds and Jongjin Park, Sungryul Kim and Songlim Kim for helpful discussion. This work was supported, in part, by grants from the Crop Functional Genomic Center, the 21st Century Frontier Program (Grant CG1111); from the Biogreen 21 Program, Rural Development Administration (20070401-034-001-007-03-00); from the Korea Research Foundation Grant (MOEHRD, Basic Research Promotion Fund (KRF-2007-341-C00028); and from Kyoung Hee University (20100791).

Supplementary material

11103_2010_9687_MOESM1_ESM.pptx (80 kb)
Supplemental Figure S1. Alignment of OsDSG1 with AIP2, via ClustalW and BoxShade programs. Conserved protein positions and RING finger motif (∇) are marked. (PPTX 79 kb)
11103_2010_9687_MOESM2_ESM.pptx (86 kb)
Supplemental Figure S2. Seed germination rates for various rice cultivars. (PPTX 85 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Gi-Gyeong Park
    • 1
    • 2
  • Jong-Jin Park
    • 3
  • Jinmi Yoon
    • 3
  • Sun-Nam Yu
    • 2
  • Gynheung An
    • 1
    Email author
  1. 1.Crop Biotech Institute & Department of Plant Molecular Systems BiotechnologyKyung Hee UniversityYonginRepublic of Korea
  2. 2.Department of Plant Genetic EngineeringCatholic University of DaeguGyeongsanRepublic of Korea
  3. 3.Department of Life SciencePohang University of Science and Technology (POSTECH)PohangRepublic of Korea

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