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Plant Molecular Biology

, Volume 73, Issue 4–5, pp 507–517 | Cite as

OsZIP5 is a plasma membrane zinc transporter in rice

  • Sichul Lee
  • Hee Joong Jeong
  • Sun A. Kim
  • Joohyun Lee
  • Mary Lou Guerinot
  • Gynheung AnEmail author
Article

Abstract

Zinc is essential for normal plant growth and development. To understand its transport in rice, we characterized OsZIP5, which is inducible under Zn deficiency. OsZIP5 complemented the growth defect of a yeast Zn-uptake mutant, indicating that OsZIP5 is a Zn transporter. The OsZIP5-GFP fusion protein was localized to the plasma membrane. Transgenic plants overexpressing the gene grew less well. Overexpression of the gene decreased the Zn concentration in shoots, but caused it to rise in the roots. Knockout plants showed no visible phenotypic changes under either normal or deficient conditions. However, they were tolerant to excess Zn and contained less Zn. In contrast, overexpressing transgenics were sensitive to excess Zn. These results indicate that OsZIP5 plays a role in Zn distribution within rice.

Keywords

OsZIP5 Overexpression Rice T-DNA tagging Zinc homeostasis 

Notes

Acknowledgments

We thank In-Soon Park and Kyungsook An for generating the transgenic lines, Yoonja Cho for managing the transgenic seeds, and Chang-Duk Jung for growing the transformed plants. This work was supported in part by grants from the Crop Functional Genomic Center, the twentyfirst Century Frontier Program (Grant CG1111); the Biogreen 21 Program (034-001-007-03-00), Rural Development Administration; the Basic Research Promotion Fund (KRF-2007-341-C00028); Kyung Hee University; and the National Science Foundation (grant no. DB10701119 to M.L.G.).

Supplementary material

11103_2010_9637_MOESM1_ESM.tif (838 kb)
Supplemental Fig. S1 Phylogenic analysis of ZIP members from Arabidopsis and rice. Protein sequences were aligned using the Clustal X and phylogenetic tree was visualized with MEGA program. Accession numbers: AB070226 for OsIRT1; AB126086 for OsIRT2; AY302058 for OsZIP1; AY302059 for OsZIP2; AY323915 for OsZIP3; AB126089 for OsZIP4; AB126087 for OsZIP5; AB126088 for OsZIP6; AB126090 for OsZIP7; AY324148 for OsZIP8; LOC_Os05g39540 for OsZIP9; LOC_Os06g37010 for OsZIP10; AAC24197 for AtZIP1; AAC24198 for AtZIP2; AAC24199 for AtZIP3; AAB65480 for AtZIP4; AAL38432 for AtZIP5; AAL38433 for AtZIP6; AAL38434 for AtZIP7; AAL83293 for AtZIP8; AAL38435 for AtZIP9; AAL38436 for AtZIP10; AAL67953 for AtZIP11; AAL38437 for AtZIP12; AAB01678 for AtIRT1; NP_001031670 for AtIRT2; NP_564766 for AtIRT3. (TIFF 838 kb)
11103_2010_9637_MOESM2_ESM.tif (1.7 mb)
Supplemental Fig. S2 Roots expressing GFP under control of CaMV 35S promoter (P35S-GFP). GFP fluorescence in control was detected throughout cytosol. Fluorescence (left) and bright-field (middle) images are overlaid at right. Bars = 10 µm. (TIFF 1710 kb)
11103_2010_9637_MOESM3_ESM.tif (43 kb)
Supplemental Fig. S3 Cu (a) and Mn (b) contents were measured from four flag leaves at flowering stage. Error bars represent standard deviation. Significant differences from WT were determined by Student’s t test, * P < 0.05. (TIFF 43 kb)
11103_2010_9637_MOESM4_ESM.tif (66 kb)
Supplemental Fig. S4 Quantification of WT and OsZIP5 mutant plants grown on Fe-deficient (a), Cu-deficient (b), and Mn-deficient (c) media for 8 days following germination. Heights of WT, oszip5-1 oszip5-2, Z5OX-5, and Z5OX-6 plants (n = 8 each) are shown. (TIFF 66 kb)
11103_2010_9637_MOESM5_ESM.tif (129 kb)
Supplemental Fig. S5 Metal-ion measurements at seedling stage. Iron concentrations in shoots and roots from WT, oszip5-1, oszip5-2, Z5OX-5, and Z5OX-6 plants grown on Fe-sufficient (a) or -deficient (b) media (n = 4 each). Cu (c) and Mn (d) concentrations in shoots and roots grown on control MS medium (n = 4 each). (TIFF 128 kb)
11103_2010_9637_MOESM6_ESM.tif (41 kb)
Supplemental Fig. S6 Concentrations of Cu (a) and Mn (b) in mature seeds. Error bars represent standard deviation. Ten seeds were pooled, with four replicates prepared. Significant differences from WT were determined by Student’s t-tests.* P < 0.05. (TIFF 41 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Sichul Lee
    • 1
    • 2
    • 3
  • Hee Joong Jeong
    • 1
    • 2
  • Sun A. Kim
    • 3
  • Joohyun Lee
    • 3
  • Mary Lou Guerinot
    • 3
  • Gynheung An
    • 1
    • 2
    Email author
  1. 1.Department of Plant Molecular Systems Biotechnology and Crop Biotech InstituteKyung Hee UniversityYonginRepublic of Korea
  2. 2.Integrative Biosciences and BiotechnologyPohang University of Science and Technology (POSTECH)PohangRepublic of Korea
  3. 3.Department of Biological SciencesDartmouth CollegeHanoverUSA

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