Plant Molecular Biology

, Volume 73, Issue 3, pp 271–281 | Cite as

SacRALF1, a peptide signal from the grass sugarcane (Saccharum spp.), is potentially involved in the regulation of tissue expansion

  • Fabiana B. Mingossi
  • Juliana L. Matos
  • Ana Paula Rizzato
  • Ane H. Medeiros
  • Maria C. Falco
  • Marcio C. Silva-Filho
  • Daniel S. Moura
Article

Abstract

Rapid alkalinization factor (RALF) is part of a growing family of small peptides with hormone characteristics in plants. Initially isolated from leaves of tobacco plants, RALF peptides can be found throughout the plant kingdom and they are expressed ubiquitously in plants. We took advantage of the small gene family size of RALF genes in sugarcane and the ordered cellular growth of the grass sugarcane leaves to gain information about the function of RALF peptides in plants. Here we report the isolation of two RALF peptides from leaves of sugarcane plants using the alkalinization assay. SacRALF1 was the most abundant and, when added to culture media, inhibited growth of microcalli derived from cell suspension cultures at concentrations as low as 0.1 μM. Microcalli exposed to exogenous SacRALF1 for 5 days showed a reduced number of elongated cells. Only four copies of SacRALF genes were found in sugarcane plants. All four SacRALF genes are highly expressed in young and expanding leaves and show a low or undetectable level of expression in expanded leaves. In half-emerged leaf blades, SacRALF transcripts were found at high levels at the basal portion of the leaf and at low levels at the apical portion. Gene expression analyzes localize SacRALF genes in elongation zones of roots and leaves. Mature leaves, which are devoid of expanding cells, do not show considerable expression of SacRALF genes. Our findings are consistent with SacRALF genes playing a role in plant development potentially regulating tissue expansion.

Keywords

Alkalinization assay Cell suspension Development Peptide hormone 

Supplementary material

11103_2010_9613_MOESM2_ESM.ppt (194 kb)
(PPT 193 kb)

References

  1. Becker JD, Boavida LC, Carneiro J, Haury M, Feijo JA (2003) Transcriptional profiling of arabidopsis tissues reveals the unique characteristics of the pollen transcriptome. Plant Physiol 133:713–725CrossRefPubMedGoogle Scholar
  2. Cheng J, Seeley KA, Sung ZR (1995) RML7 and RML2, Arabidopsis genes required for cell proliferation at the root tip. Plant Physiol 107:365–376CrossRefPubMedGoogle Scholar
  3. Dickison WC (2000) Integrative plant anatomy. Academic Press, New YorkGoogle Scholar
  4. Escobar NM, Haupt S, Thow G, Boevink P, Chapman S, Oparka K (2003) High-throughput viral expression of cDNA-green fluorescent protein fusions reveals novel subcellular addresses and identifies unique proteins that interact with plasmodesmata. Plant Cell 15:1507–1523CrossRefPubMedGoogle Scholar
  5. Falco MC, Tulmann Neto A, Mendes BMJ, Zapata-Arias FJ (1996) Isolation and cultivation of sugarcane protoplasts. Braz J Plant Physiol 8:175–179Google Scholar
  6. Gendreau E, Traas J, Desnos T, Grandjean O, Caboche M, Höfte H (1997) Cellular basis of hypocotyl growth in Arabidopsis thaliana. Plant Physiol 114:295–305CrossRefPubMedGoogle Scholar
  7. Germain H, Chevalier E, Caron S, Matton DP (2005) Characterization of five RALF-like from Solanun chacoense provides support for a developmental role in plants. Planta 220:447–454CrossRefPubMedGoogle Scholar
  8. Grivet L, Arruda P (2002) Sugarcane genomics: depicting the complex genome of an important tropical crop. Curr Opin Plant Biol 5:122–127CrossRefPubMedGoogle Scholar
  9. Haruta M, Constabel CP (2003) Rapid alkalinization factors in poplar cell cultures. Peptide isolation, cDNA cloning, and differential expression in leaves and methyl jasmonate-treated cells. Plant Physiol 131:814–823CrossRefPubMedGoogle Scholar
  10. Haruta M, Monshausen G, Gilroy S, Sussman MR (2008) A Cytoplasmic Ca2+ functional assay for identifying and purifying endogenous cell signaling peptides in Arabidopsis seedlings: identification of AtRALF1 peptide. Biochemistry 47:6311–6321CrossRefPubMedGoogle Scholar
  11. Iskandar HM, Simpson RS, Casu RE, Bonnett GD, Maclean DJ, Manners JM (2004) Comparison of reference genes for quantitative real-time polymerase chain reaction analysis of gene expression in sugarcane. Plant Mol Biol Rep 22:325–337CrossRefGoogle Scholar
  12. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefPubMedGoogle Scholar
  13. Matos JM, Fiori CS, Silva-Filho MC, Moura DS (2008) A conserved dibasic site is essential for correct processing of the peptide hormone AtRALF1 in Arabidopsis thaliana. FEBS Lett 582:3343–3347CrossRefPubMedGoogle Scholar
  14. McCubbin AG, Lee C, Hetrick A (2006) Identification of genes showing differential expression between morphs in developing flowers of Primula vulgaris. Sex Plant Reprod 19:63–72CrossRefGoogle Scholar
  15. Moore PH (1987) Anatomy and morphology. In: Heinz DJ (ed) Sugarcane improvement through breeding. Elsevier Science, New York, pp 85–143Google Scholar
  16. Olsen AN, Mundy J, Skriver K (2002) Peptomics, identification of novel cationic Arabidopsis peptides with conserved sequence motifs. In Silico Biol 2:441–451PubMedGoogle Scholar
  17. Pearce G, Ryan CA (2003) Systemic signaling in tomato plants for defense against herbivores. Isolation and characterization of three novel defense-signaling glycopeptide hormones coded in a single precursor gene. J Biol Chem 278:30044–30050CrossRefPubMedGoogle Scholar
  18. Pearce G, Moura DS, Stratmann J, Ryan CA (2001a) RALF, a 5-kDa ubiquitous polypeptide in plants, arrests root growth and development. Proc Natl Acad Sci USA 98:12843–12847CrossRefPubMedGoogle Scholar
  19. Pearce G, Moura DS, Stratmann J, Ryan CA (2001b) Production of multiple plant hormones from a single polyprotein precursor. Nature 411:817–820CrossRefPubMedGoogle Scholar
  20. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:2001–2007CrossRefGoogle Scholar
  21. Scheer JM, Pearce G, Ryan CA (2005) LeRALF, a plant peptide that regulates root growth and development, specifically binds to 25 and 120 kDa cell surface membrane proteins of Lycopersicon peruvianum. Planta 221:667–674CrossRefPubMedGoogle Scholar
  22. Silverstein KA, Graham MA, VandenBosch KA (2006) Novel paralogous gene families with potential function in legume nodules and seeds. Curr Opin Plant Biol 9:142–146CrossRefPubMedGoogle Scholar
  23. Srivastava R, Liu J, Guo H, Yin Y, Howell SH (2009) Regulation and processing of a plant peptide hormone, AtRALF23, in Arabidopsis. Plant J 59:930–939CrossRefPubMedGoogle Scholar
  24. Vettore AL, da Silva FR, Kemper EL, Souza GM, da Silva AM, Ferro MI, Henrique-Silva F, Giglioti EA, Lemos MV, Coutinho LL, Nobrega MP, Carrer H, França SC, Bacci Júnior M, Goldman MH, Gomes SL, Nunes LR, Camargo LE, Siqueira WJ, Van Sluys MA, Thiemann OH, Kuramae EE, Santelli RV, Marino CL, Targon ML, Ferro JA, Silveira HC, Marini DC, Lemos EG, Monteiro-Vitorello CB, Tambor JH, Carraro DM, Roberto PG, Martins VG, Goldman GH, de Oliveira RC, Truffi D, Colombo CA, Rossi M, de Araujo PG, Sculaccio SA, Angella A, Lima MM, de Rosa Júnior VE, Siviero F, Coscrato VE, Machado MA, Grivet L, Di Mauro SM, Nobrega FG, Menck CF, Braga MD, Telles GP, Cara FA, Pedrosa G, Meidanis J, Arruda P (2003) Analysis and functional annotation of an expressed sequence tag collection for tropical crop sugarcane. Genome Res 13:2725–2735CrossRefPubMedGoogle Scholar
  25. Weigel D, Glazebrook J (2002) Arabidopsis: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  26. Wold F (1981) In vivo chemical modification of proteins (post-translational modification). Annu Rev Biochem 50:783–814CrossRefPubMedGoogle Scholar
  27. Wu J, Kurten EL, Monshausen G, Hummel GM, Gilroy S, Baldwin IT (2007) NaRALF, a peptide signal essential for the regulation of root hair tip apoplastic pH in Nicotiana attenuata, is required for root hair development and plant growth in native soils. Plant J 52:877–890CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Fabiana B. Mingossi
    • 2
  • Juliana L. Matos
    • 1
  • Ana Paula Rizzato
    • 2
    • 4
  • Ane H. Medeiros
    • 2
  • Maria C. Falco
    • 3
  • Marcio C. Silva-Filho
    • 2
  • Daniel S. Moura
    • 1
  1. 1.Departamento de Ciências Biológicas, Escola Superior de Agricultura Luiz de QueirozUniversidade de São PauloPiracicabaBrazil
  2. 2.Departamento de Genética, Escola Superior de Agricultura Luiz de QueirozUniversidade de São PauloPiracicabaBrazil
  3. 3.Centro de Tecnologia Canavieira, CTCPiracicabaBrazil
  4. 4.Alellyx Applied GenomicsCampinasBrazil

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