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Plant Molecular Biology

, Volume 73, Issue 1–2, pp 169–179 | Cite as

Characterization, expression and function of DORMANCY ASSOCIATED MADS-BOX genes from leafy spurge

  • David P. Horvath
  • Sibum Sung
  • Donghwan Kim
  • Wun Chao
  • James Anderson
Article

Abstract

DORMANCY ASSOCIATED MADS-BOX (DAM) genes are related to AGAMOUS-LIKE 24 and SHORT VEGETATIVE PHASE genes of arabidopsis and are differentially regulated coordinately with endodormancy induction and release in buds of several perennial plant species. DAM genes were first shown to directly impact endodormancy in peach where a deletion of a series of DAM resulted in loss of endodormancy induction. We have cloned and characterized several MADS box genes from the model perennial weed leafy spurge. Leafy spurge DAM genes are preferentially expressed in shoot tips and buds in response to cold temperatures and day length in a manner that is relative to the level of endodormancy induced by various environmental conditions. Over-expression of one DAM gene in arabidopsis delays flowering. Additionally, we show that at least one DAM gene is differentially regulated by chromatin remodeling. Comparisons of the DAM gene promoters between poplar and leafy spurge have identified several conserved sequences that may be important for their expression patterns in response to dormancy-inducing stimuli.

Keywords

Endodormancy MADS-box Phylogenetic footprinting Flowering 

Notes

Acknowledgments

I would like to thank Laura Kelly for significant technical assistance with these experiments and Dr. Marion Wood for her helpful commentary on this manuscript.

Supplementary material

11103_2009_9596_MOESM1_ESM.doc (48 kb)
(DOC 48 kb)
11103_2009_9596_MOESM2_ESM.doc (37 kb)
(DOC 37 kb)

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Copyright information

© U.S. Government 2010

Authors and Affiliations

  • David P. Horvath
    • 1
  • Sibum Sung
    • 2
  • Donghwan Kim
    • 2
  • Wun Chao
    • 1
  • James Anderson
    • 1
  1. 1.Biosciences Research LaboratoryUSDA-ARSFargoUSA
  2. 2.Section of Molecular Cell & Developmental Biology, School of Biological Sciences, Institute for Cellular and Molecular BiologyThe University of Texas at AustinAustinUSA

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