Expression of vacuolar H+-pyrophosphatase (OVP3) is under control of an anoxia-inducible promoter in rice
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Vacuolar H+-pyrophosphatase (V-PPase) expression increases in a number of abiotic stresses and is thought to play a role in adaptation to abiotic stresses. This paper reports on the regulation of six V-PPase genes in rice (Oryza sativa L.) coleoptiles under anoxia, using flood tolerant and intolerant cultivars to test our hypothesis. Quantitative PCR analysis showed that one vacuolar H+-pyrophosphatase (OVP3) was induced by anoxia, particularly in flood-tolerant rice. Regulation of OVP3 expression under anoxia was investigated by analysing putative OVP promoters. The putative OVP3 promoter contained more previously identified anoxia-inducible motifs than the putative promoters of the other five OVP genes. GUS activity in transgenic rice plants containing the OVP3 promoter region linked to the GUS reporter gene was induced only by anoxia. Salt and cold treatments had little effect on OVP3 promoter-driven GUS expression when compared to the anoxic treatment.
KeywordsAnoxia Gene expression Promoter Rice Vacuolar H+-pyrophosphatase
We thank Olivier Cotsaftis for expert assistance with rice transformation, Andrew Harvey for sequence analysis, Ezaz Mamun for vacuole isolation and Gwenda Mayo for technical support involving microscopy. We are also grateful to Masayoshi Maeshima (Nagoya University, Japan) for the gift of the anti-V-PPase and anti-V-ATPase antibodies. This research was made possible through the generosity of the Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Australia.
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