Plant Molecular Biology

, Volume 71, Issue 6, pp 585–597 | Cite as

An abundant and heavily truncated non-LTR retrotransposon (LINE) family in Beta vulgaris

  • Torsten Wenke
  • Daniela Holtgräwe
  • Axel V. Horn
  • Bernd Weisshaar
  • Thomas Schmidt
Article

Abstract

We describe a non-LTR retrotransposon family, BvL, of the long interspersed nuclear elements L1 clade isolated from sugar beet (Beta vulgaris). Characteristic molecular domains of three full-length BvL elements were determined in detail, showing that coding sequences are interrupted and most likely non-functionally. In addition, eight highly conserved endonuclease regions were defined by comparison with other plant LINEs. The abundant BvL family is widespread within the genus Beta, however, the vast majority of BvL copies are extremely 5′ truncated indicating an error-prone reverse transcriptase activity. The dispersed distribution of BvL copies on all sugar beet chromosomes with exclusion of most heterochromatic regions was shown by fluorescent in situ hybridization. The analysis of BvL 3′ end sequences and corresponding flanking regions, respectively, revealed the preferred integration of BvL into A/T-rich regions of the sugar beet genome, but no specific target sequences.

Keywords

Beta vulgaris Sugar beet Long interspersed nuclear elements Non-LTR retrotransposon Fluorescent in situ hybridization 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Torsten Wenke
    • 1
  • Daniela Holtgräwe
    • 2
  • Axel V. Horn
    • 1
  • Bernd Weisshaar
    • 2
  • Thomas Schmidt
    • 1
    • 3
  1. 1.Institute of BotanyDresden University of TechnologyDresdenGermany
  2. 2.Institute of Genome ResearchUniversity of BielefeldBielefeldGermany
  3. 3.Department of BiologyDresden University of TechnologyDresdenGermany

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