Plant Molecular Biology

, Volume 70, Issue 5, pp 499–509

Genetic features of a pollen-part mutation suggest an inhibitory role for the Antirrhinum pollen self-incompatibility determinant

  • Yongbiao Xue
  • Yijing Zhang
  • Qiuying Yang
  • Qun Li
  • Zhukuan Cheng
  • Hugh G. Dickinson
Article

Abstract

Self-incompatibility (SI), an important barrier to inbreeding in flowering plants, is controlled in many species by a single polymorphic S-locus. In the Solanaceae, two tightly linked S-locus genes, S-RNase and SLF (S-locus F-box)/SFB (S-haplotype-specific F-box), control SI expression in pistil and pollen, respectively. The pollen S-determinant appears to function to inhibit all but self S-RNase in the Solanaceae, but its genetic function in the closely-related Plantaginaceae remains equivocal. We have employed transposon mutagenesis in a member of the Plantaginaceae (Antirrhinum) to generate a pollen-part SI-breakdown mutant Pma1 (Pollen-part mutation in Antirrhinum1). Molecular genetic analyses showed that an extra telocentric chromosome containing AhSLF-S1 is present in its self-compatible but not in its SI progeny. Furthermore, analysis of the effects of selection revealed positive selection acting on both SLFs and SFBs, but with a stronger purifying selection on SLFs. Taken together, our results suggest an inhibitor role of the pollen S in the Plantaginaceae (as represented by Antirrhinum), similar to that found in the Solanaceae. The implication of these findings is discussed in the context of S-locus evolution in flowering plants.

Keywords

Self-incompatibility Antirrhinum SLF Pollen-part mutation S-RNase 

Supplementary material

11103_2009_9487_MOESM1_ESM.doc (7.7 mb)
Supplementary material 1 (DOC 7927 kb)

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Yongbiao Xue
    • 1
  • Yijing Zhang
    • 1
    • 4
  • Qiuying Yang
    • 1
    • 4
  • Qun Li
    • 1
  • Zhukuan Cheng
    • 2
  • Hugh G. Dickinson
    • 3
  1. 1.Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental BiologyChinese Academy of Sciences and National Center for Plant Gene ResearchChaoyang District, BeijingChina
  2. 2.The State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental BiologyChinese Academy of Sciences and National Center for Plant Gene ResearchBeijingChina
  3. 3.Department of Plant SciencesUniversity of OxfordOxfordUK
  4. 4.Graduate University of Chinese Academy of SciencesBeijingChina

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