Plant Molecular Biology

, Volume 70, Issue 4, pp 385–401 | Cite as

Conserved and novel miRNAs in the legume Phaseolus vulgaris in response to stress

  • Catalina Arenas-Huertero
  • Beatriz Pérez
  • Fernando Rabanal
  • Daniel Blanco-Melo
  • Carlos De la Rosa
  • Georgina Estrada-Navarrete
  • Federico Sanchez
  • Alejandra Alicia Covarrubias
  • José Luis Reyes
Article

Abstract

MicroRNAs (miRNAs) are small RNA molecules recognized as important regulators of gene expression. Although plant miRNAs have been extensively studied in model systems, less is known in other plants with limited genome sequence data. We are interested in the identification of miRNAs in Phaseolus vulgaris (common bean) to uncover different plant strategies to cope with adverse conditions and because of its relevance as a crop in developing countries. Here we present the identification of conserved and candidate novel miRNAs in P. vulgaris present in different organs and growth conditions, including drought, abscisic acid treatment, and Rhizobium infection. We also identified cDNA sequences in public databases that represent the corresponding miRNA precursors. In addition, we predicted and validated target mRNAs amongst reported EST and cDNAs for P. vulgaris. We propose that the novel miRNAs present in common bean and other legumes, are involved in regulation of legume-specific processes including adaptation to diverse external cues.

Keywords

miRNAs Water deficit Glycine max Medicago truncatula Common bean Abscisic acid 

Notes

Acknowledgments

We thank the help of Jerome Verleyen and Roberto Rodríguez during the early phases of sequence analysis. This work was supported by grants from CONACyT, Mexico (J48740) and DGAPA-UNAM (IN-227706) to JLR, and JLR and AAC, respectively.

Supplementary material

11103_2009_9480_MOESM1_ESM.xls (24 kb)
Pre-miRNAs for conserved P. vulgaris miRNAs (XLS 24 kb)
11103_2009_9480_MOESM2_ESM.xls (48 kb)
Predicted miRNA targets for conserved miRNAs in P. vulgaris (XLS 48 kb)
11103_2009_9480_MOESM3_ESM.pdf (7.6 mb)
(PDF 7762 kb)

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Catalina Arenas-Huertero
    • 1
  • Beatriz Pérez
    • 1
  • Fernando Rabanal
    • 1
  • Daniel Blanco-Melo
    • 1
  • Carlos De la Rosa
    • 1
  • Georgina Estrada-Navarrete
    • 1
  • Federico Sanchez
    • 1
  • Alejandra Alicia Covarrubias
    • 1
  • José Luis Reyes
    • 1
  1. 1.Departamento de Biología Molecular de Plantas, Instituto de BiotecnologíaUniversidad Nacional Autónoma de MéxicoCuernavacaMexico

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