A novel extended family of stromal thioredoxins
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Thioredoxins play key regulatory roles in chloroplasts by linking photosynthetic light reactions to a series of plastid functions. In addition to the established groups of thioredoxins, f, m, x, and y, novel plant thioredoxins were also considered to include WCRKC motif proteins, CDSP32, the APR proteins, the lilium proteins and HCF164. Despite their important roles, the subcellular locations of many novel thioredoxins has remained unknown. Here, we report a study of their subcellular location using the cDNA clone resources of TAIR. In addition to filling all gaps in the subcellular map of the established chloroplast thioredoxins f, m, x and y, we show that the members of the WCRKC family are targeted to the stroma and provide evidence for a stromal location of the lilium proteins. The combined data from this and related studies indicate a consistent stromal location of the known Arabidopsis chloroplast thioredoxins except for thylakoid-bound HCF164.
KeywordsArabidopsis thaliana Chloroplast Protein targeting Subcellular location Thioredoxin
This study was supported by grants from the J. C. Kempe Memorial Fund Foundation (M. H.), the Carl-Trygger Foundation (T. K.) and the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS, to W. P. S).
- Bartsch S, Monnet J, Selbach K, Quigley F, Gray J, von Wettstein D, Reinbothe S, Reinbothe C (2008) Three thioredoxin targets in the inner envelope membrane of chloroplasts function in protein import and chlorophyll metabolism. Proc Natl Acad Sci USA 105:4933–4938. doi:10.1073/pnas.0800378105 PubMedCrossRefGoogle Scholar
- Brock IW, Hazell L, Michl D, Nielsen VS, Moller BL, Herrmann RG, Klosgen RB, Robinson C (1993) Precursors of one integral and five lumenal thylakoid proteins are imported by isolated pea and barley thylakoids: optimisation of in vitro assays. Plant Mol Biol 23:717–725. doi:10.1007/BF00021527 PubMedCrossRefGoogle Scholar
- Buchanan BB (1980) Role of light in the regulation of chloroplast enzymes. Annu Rev Plant Physiol Plant Mol Biol 31:341–374Google Scholar
- Dangoor I, Peled-Zehavi H, Levitan A, Pasand O, Danon A (2008) A small family of chloroplast atypical thioredoxins. Plant Physiol. doi:10.1104/pp.108.128314
- Friso G, Giacomelli L, Ytterberg AJ, Peltier JB, Rudella A, Sun Q, Wijk KJ (2004) In-depth analysis of the thylakoid membrane proteome of Arabidopsis thaliana chloroplasts: new proteins, new functions, and a plastid proteome database. Plant Cell 16:478–499. doi:10.1105/tpc.017814 PubMedCrossRefGoogle Scholar
- Gopalan G, He Z, Balmer Y, Romano P, Gupta R, Heroux A, Buchanan BB, Swaminathan K, Luan S (2004) Structural analysis uncovers a role for redox in regulating FKBP13, an immunophilin of the chloroplast thylakoid lumen. Proc Natl Acad Sci USA 101:13945–13950. doi:10.1073/pnas.0405240101 PubMedCrossRefGoogle Scholar
- Hall M, Schröder WP, Kieselbach T (2008) Thioredoxin interactions of the chloroplast lumen of Arabidopsis thaliana indicate a redox regulation of the xanthophyll cycle. In: Allen JF, Gantt JH, Osmond B (eds) Photosynthesis. Energy from the sun—14th international congress on photosynthesis, Springer, Netherlands, pp 1099–1102. doi:10.1007/978-1-4020-6709-9 CrossRefGoogle Scholar
- Yamada K, Lim J, Dale JM, Chen H, Shinn P, Palm CJ, Southwick AM, Wu HC, Kim C, Nguyen M, Pham P, Cheuk R, Karlin-Newmann G, Liu SX, Lam B, Sakano H, Wu T, Yu G, Miranda M, Quach HL, Tripp M, Chang CH, Lee JM, Toriumi M, Chan MM, Tang CC, Onodera CS, Deng JM, Akiyama K, Ansari Y, Arakawa T, Banh J, Banno F, Bowser L, Brooks S, Carninci P, Chao Q, Choy N, Enju A, Goldsmith AD, Gurjal M, Hansen NF, Hayashizaki Y, Johnson-Hopson C, Hsuan VW, Iida K, Karnes M, Khan S, Koesema E, Ishida J, Jiang PX, Jones T, Kawai J, Kamiya A, Meyers C, Nakajima M, Narusaka M, Seki M, Sakurai T, Satou M, Tamse R, Vaysberg M, Wallender EK, Wong C, Yamamura Y, Yuan S, Shinozaki K, Davis RW, Theologis A, Ecker JR (2003) Empirical analysis of transcriptional activity in the Arabidopsis genome. Science 302:842–846. doi:10.1126/science.1088305 PubMedCrossRefGoogle Scholar