Plant Molecular Biology

, Volume 70, Issue 1–2, pp 173–192

Transcriptome analysis during somatic embryogenesis of the tropical monocot Elaeisguineensis: evidence for conserved gene functions in early development

  • Hsiang-Chun Lin
  • Fabienne Morcillo
  • Stéphane Dussert
  • Christine Tranchant-Dubreuil
  • James W. Tregear
  • Timothy John Tranbarger


With the aim of understanding the molecular mechanisms underlying somatic embryogenesis (SE) in oil palm, we examined transcriptome changes that occur when embryogenic suspension cells are initiated to develop somatic embryos. Two reciprocal suppression subtractive hybridization (SSH) libraries were constructed from oil palm embryogenic cell suspensions: one in which embryo development was blocked by the presence of the synthetic auxin analogue 2,4-dichlorophenoxyacetic acid (2,4-d) in the medium (proliferation library); and another in which cells were stimulated to form embryos by the removal of 2,4-d from the medium (initiation library). A total of 1867 Expressed Sequence Tags (ESTs) consisting of 1567 potential unigenes were assembled from the two libraries. Functional annotation indicated that 928 of the ESTs correspond to proteins that have either no similarity to sequences in public databases or are of unknown function. Gene Ontology (GO) terms assigned to the two EST populations give clues to the underlying molecular functions, biological processes and cellular components involved in the initiation of embryo development. Macroarrays were used for transcript profiling the ESTs during SE. Hierarchical cluster analysis of differential transcript accumulation revealed 4 distinct profiles containing a total of 192 statistically significant developmentally regulated transcripts. Similarities and differences between the global results obtained with in vitro systems from dicots, monocots and gymnosperms will be discussed.


Somatic embryogenesis Oil palm Auxin Transcript profiling 



2,4-Dichlorophenoxyacetic acid


ADP-ribosylation factor


Expressed sequence tag


Gene Ontology


Glutathione S-transferases


Hierarchical cluster analysis






Somatic embryo


Somatic embryogenesis


Suppression subtractive hybridization

Supplementary material

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Hsiang-Chun Lin
    • 1
    • 4
  • Fabienne Morcillo
    • 2
  • Stéphane Dussert
    • 3
  • Christine Tranchant-Dubreuil
    • 3
  • James W. Tregear
    • 1
  • Timothy John Tranbarger
    • 1
  1. 1.IRD, UMR DIAPC, IRD/CIRAD Palm Development GroupMontpellier Cedex 5France
  2. 2.CIRAD, UMR DIAPC, IRD/CIRAD Palm Development GroupMontpellier Cedex 5France
  3. 3.IRD, UMR DIAPCMontpellier Cedex 5France
  4. 4.ETH ZurichInstitute of Plant ScienceZurichSwitzerland

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