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Plant Molecular Biology

, 68:505 | Cite as

Import of preproteins into the chloroplast inner envelope membrane

  • Ewa Firlej-Kwoka
  • Penelope Strittmatter
  • Jürgen Soll
  • Bettina BölterEmail author
Article

Abstract

The chloroplast inner envelope membrane contains many integral proteins which differ in the number of α-helices that anchor the protein into the bilayer. For most of these proteins it is not known which pathway they engage to reach their final localisation within the membrane. In yeast mitochondria, two distinct sorting/insertion pathways have been described for integral inner membrane proteins, involving the Tim22 and Tim23 translocases. These routes involve on the one hand a conservative sorting, on the other hand a stop-transfer pathway. In this study we performed a systematic characterisation of the import behaviour of seven inner envelope proteins representing different numbers of predicted α-helices. We investigated their energy dependence, import rate, involvement of components of the chloroplast general import pathway and distribution between soluble and membrane fractions. Our results show the existence of at least two different families of inner envelope proteins that can be classified due to the occurrence of an intermediate processing form. Each of the proteins we investigated seems to use a stop-transfer pathway for insertion into the inner envelope.

Keywords

Chloroplast Protein import Inner envelope membrane Stop-transfer 

Abbreviations

ATP

Adenosinetriphosphate

Erv1

Essential for respiration and vegetative growth 1

HP

Hypothetical protein

IEP

Inner envelope protein

Lep

Leader peptidase

Mia40

Mitochondrial IMS import and assembly protein of 40 kDa

mOE33

Mature form of the oxygen evolving complex protein of 33 kDa

PIC1

Permease in chloroplasts 1

Plsp1

Plastidic signal peptidase type I protease 1

pOE33

Precursor of the oxygen evolving complex protein of 33 kDa

PPT

Phosphoenolpyruvate translocator

pSSU

Precursor of the small subunit of the Ribulosebisphosphatecarboxylase/oxygenase

Sam

Sorting and assembly machinery

SSP

Stromal processing peptidase

Tic

Translocon at the inner envelope of chloroplasts

Tim4

Translocation intermediate 4

Tim

Translocase of the inner mitochondrial membrane

TL

Translation product

Toc

Translocon at the outer envelope of chloroplasts

Notes

Acknowledgements

We acknowledge funding by the DFG (P. S., J. S. and B. B.) and Bayhost (E. F.-K.) and we thank Prof. Bob Buchanan for critical reading of the manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Ewa Firlej-Kwoka
    • 1
    • 2
  • Penelope Strittmatter
    • 1
    • 2
  • Jürgen Soll
    • 1
    • 2
  • Bettina Bölter
    • 1
    • 2
    Email author
  1. 1.Department Biology I, Plant BiochemistryLudwig-Maximilians-Universität MünchenPlanegg-MartinsriedGermany
  2. 2.Munich Center for Integrated Protein Science CiPSMLudwig-Maximilians-Universität MünchenMunichGermany

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