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Plant Molecular Biology

, Volume 68, Issue 1–2, pp 105–117 | Cite as

Lack of repeatable differential expression patterns between MON810 and comparable commercial varieties of maize

  • Anna Coll
  • Anna Nadal
  • Montserrat Palaudelmàs
  • Joaquima Messeguer
  • Enric Melé
  • Pere Puigdomènech
  • Maria PlaEmail author
Article
First Online:

Abstract

The introduction of genetically modified organisms (GMO) in many countries follows strict regulations to assure that only products that have been safety tested in relation to human health and the environment are marketed. Thus, GMOs must be authorized before use. By complementing more targeted approaches, profiling methods can assess possible unintended effects of transformation. We used microarrays to compare the transcriptome profiles of widely commercialized maize MON810 varieties and their non-GM near-isogenic counterparts. The expression profiles of MON810 seedlings are more similar to those of their corresponding near-isogenic varieties than are the profiles of other lines produced by conventional breeding. However, differential expression of ∼1.7 and ∼0.1% of transcripts was identified in two variety pairs (AristisBt/Aristis and PR33P67/PR33P66) that had similar cryIA(b) mRNA levels, demonstrating that commercial varieties of the same event have different similarity levels to their near-isogenic counterparts without the transgene (note that these two pairs also show phenotypic differences). In the tissues, developmental stage and varieties analyzed, we could not identify any gene differentially expressed in all variety-pairs. However, a small set of sequences were differentially expressed in various pairs. Their relation to the transgenesis was not proven, although this is likely to be modulated by the genetic background of each variety.

Keywords

GMO (Genetically Modified Organism) MON810 Maize Transcriptome Unintended effects Expression profile 

Abbreviations

cDNA

Complementary DNA

CRM

Certified reference material

E

Efficiency

EBI

European Bioinformatics Institute

EFSA

European Food Safety Authority

EU

European Union

FAO/WHO

Food and Agriculture Organization / World Health Organization

GM

Genetically Modified

GMO

Genetically Modified Organism

IRMM

Institute for Reference Materials and Measurements

ISAAA

International Service for the Acquisition of Agri-biotech Applications

mRNA

messenger RNA

OECD

Organisation for Economic Co-operation and Development

real-time RT-PCR

Reverse transcription—real-time polymerase chain reaction

RMA

Robust Multichip Average

rRNA

ribosomal RNA

V2

Vegetative two-leaf stage

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Notes

Acknowledgements

We specially thank Prof. E. Montesinos (INTEA, UdG) for critically reading of the manuscript. We thank R. Collado (UdG) and J.M. García-Cantalejo (Parque Científico de Madrid) for technical assistance; T. Esteve (CRAG), J. Serra and J. Salvia (E.E.A. Mas Badia) for valuable suggestions. This work was financially supported by the Spanish MEC project with ref. AGL2007-65903/AGR. AC received a studentship from the Generalitat de Catalunya (2005FI 00144).

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Anna Coll
    • 1
  • Anna Nadal
    • 2
  • Montserrat Palaudelmàs
    • 3
  • Joaquima Messeguer
    • 3
  • Enric Melé
    • 3
  • Pere Puigdomènech
    • 2
  • Maria Pla
    • 1
    Email author
  1. 1.Institut de Tecnologia Agroalimentària (INTEA)Universitat de GironaGironaSpain
  2. 2.Departament Genètica Molecular, Centre de Recerca en Agrigenòmica CSIC-IRTA-UABBarcelonaSpain
  3. 3.Departament Genètica Vegetal, Centre de Recerca en AgrigenòmicaCSIC-IRTA-UABBarcelonaSpain

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