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Plant Molecular Biology

, Volume 67, Issue 1–2, pp 37–55 | Cite as

Identification of early senescence-associated genes in rice flag leaves

  • Li Liu
  • Yong Zhou
  • Gang Zhou
  • Rongjian Ye
  • Lina Zhao
  • Xianghua Li
  • Yongjun LinEmail author
Article

Abstract

Leaf senescence is one of the key stages of plant leaf development. It is a highly complex but ordered process involving expression of large scale senescence associated genes, and its molecular mechanisms still remain unclear. By using suppression subtractive hybridization, 815 ESTs that are up-regulated at the onset of rice flag leaf senescence have been isolated. A total of 533 unigenes have been confirmed by macroarray detection and sequencing. 183 of these unigenes have GO annotations, involved in macromolecule metabolism, protein biosynthesis regulation, energy metabolism, gene expression regulations, detoxification, pathogenicity and stress, cytoskeleton organization and flower development. Another 121 unigenes co-localized with previously reported known stay-green QTLS. RT-PCR analysis on the other novel genes indicated that they can be up-regulated in natural early senescence and induced by hormone. Our results indicate that senescence is closely related to various metabolic pathways, thus providing new insight into the onset of leaf senescence mechanism.

Keywords

Leaf senescence Macroarray Rice Senescence-associated genes (SAGs) Suppression subtractive hybridization (SSH) 

Abbreviations

ABA

Abscisic acid

ACC

1-Amino-cyclopropane-1-carboxylic acid

GA

Gibberellin acid

GO

Gene ontology

HR

Hypersensitive response

KT

Kinetin

PCD

Programmed cell death

ROS

Reactive oxygen species

SAGs

Senescence associated genes

SSH

Suppression subtractive hybridization

Notes

Acknowledgements

This research was funded by the National Program on Research and Development of Transgenic Plants, the National Natural Science Foundation of China and the National High Technology Research and Development Program of China (863 Program). We sincerely thank PhD students Meng Cai in National Center of Plant Gene Research for discussion, and PhD student Yibo Li for providing the molecular linkage map.

Supplementary material

11103_2008_9300_MOESM1_ESM.xls (50 kb)
(XLS 50 kb)

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Li Liu
    • 1
  • Yong Zhou
    • 1
  • Gang Zhou
    • 1
  • Rongjian Ye
    • 1
  • Lina Zhao
    • 1
  • Xianghua Li
    • 1
  • Yongjun Lin
    • 1
    Email author
  1. 1.National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene ResearchHuazhong Agricultural UniversityWuhanP.R. China

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