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Plant Molecular Biology

, Volume 66, Issue 1–2, pp 47–59 | Cite as

The Arabidopsis SET-domain protein ASHR3 is involved in stamen development and interacts with the bHLH transcription factor ABORTED MICROSPORES (AMS)

  • Tage Thorstensen
  • Paul E. Grini
  • Inderjit S. Mercy
  • Vibeke Alm
  • Sigrid Erdal
  • Rein Aasland
  • Reidunn B. AalenEmail author
Article

Abstract

The Arabidopsis thaliana genome contains more than 30 genes encoding SET-domain proteins that are thought to be epigenetic regulators of gene expression and chromatin structure. SET-domain proteins can be divided into subgroups, and members of the Polycomb group (PcG) and trithorax group (trxG) have been shown to be important regulators of development. Both in animals and plants some of these proteins are components of multimeric protein complexes. Here, we have analyzed the Arabidopsis trxG protein ASHR3 which has a SET domain and pre- and post-SET domains similar to that of Ash1 in Drosophila. In addition to the SET domain, a divergent PHD finger is found in the N-terminus of the ASHR3 protein. As expected from SET-domain proteins involved in transcriptional activation, ASHR3 (coupled to GFP) localizes to euchromatin. A yeast two-hybrid screening revealed that the ASHR3 protein interacts with the putative basic helix-loop-helix (bHLH) transcription factor ABORTED MICROSPORES (AMS), which is involved in anther and stamen development in Arabidopsis. Deletion mapping indicated that both the PHD finger and the SET domain mediate the interaction between the two proteins. Overexpression of ASHR3 led in general to growth arrest, and specifically to degenerated anthers and male sterility. Expression analyses demonstrated that ASHR3 like AMS is expressed in the anther and in stamen filaments. We therefore propose that AMS can target ASHR3 to chromatin and regulate genes involved in stamen development and function.

Keywords

Protein-protein interaction Stamen development Over-expression phenotype 

Abbreviations

AMS

ABORTED MICROSPORES

Y2H

Yeast two hybrid

GFP

Green fluorescent protein

bHLH

Basic helix-loop-helix

Notes

Acknowledgments

Thanks to Susanne Opravil in Thomas Jenuwein’s lab for providing recombinant nucleosomes. Many thanks to B. Sæther, R. Falleth, and Solveig H. Engebretsen for technical assistance. The Research Council of Norway has supported this work and V.A. (grant 146652/431), P.E.G. (grant 166057/V40) and I.S.M. (grant 129525/420). The work was facilitated by the services provided by the Norwegian Arabidopsis Research Centre (NARC, http://www.narc.no/), a part of the Research Council of Norway’s National Programme for Research in Functional Genomics (FUGE).

Open Access

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Copyright information

© The Author(s) 2007

Authors and Affiliations

  • Tage Thorstensen
    • 1
  • Paul E. Grini
    • 1
  • Inderjit S. Mercy
    • 1
    • 2
  • Vibeke Alm
    • 1
  • Sigrid Erdal
    • 3
  • Rein Aasland
    • 3
  • Reidunn B. Aalen
    • 1
    Email author
  1. 1.Department of Molecular BiosciencesUniversity of OsloOsloNorway
  2. 2.The Norwegian School of Veterinarian ScienceOsloNorway
  3. 3.Department of Molecular BiologyUniversity of BergenBergenNorway

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