Plant Molecular Biology

, Volume 65, Issue 5, pp 615–626

Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1

  • Inna Lermontova
  • Veit Schubert
  • Frederik Börnke
  • Jiri Macas
  • Ingo Schubert
Article

DOI: 10.1007/s11103-007-9226-z

Cite this article as:
Lermontova, I., Schubert, V., Börnke, F. et al. Plant Mol Biol (2007) 65: 615. doi:10.1007/s11103-007-9226-z

Abstract

The conserved protein CBF5, initially regarded as a centromere binding protein in yeast and higher plants, was later found within nucleoli and in Cajal bodies of yeast and metazoa. There, it is assumed to be involved in posttranscriptional pseudouridinylation of various RNA species that might be important for RNA processing. We found EYFP-labeled CBF5 of A. thaliana to be located within nucleoli and Cajal bodies, but neither at centromeres nor somewhere else on chromosomes. Arabidopsis mutants carrying a homozygous T-DNA insertion at the CBF5 locus were lethal. Yeast two-hybrid and mRNA expression analyses demonstrated that AtCBF5 is co-expressed and interacts with a previously uncharacterized protein containing a conserved NAF1 domain, presumably involved in H/ACA box snoRNP biogenesis. The homologous yeast protein has been shown to contribute to RNA pseudouridinylation. Thus, AtCBF5 might have an essential function in RNA processing rather than being a kinetochore protein.

Keywords

Cajal bodies Centromere H/ACA snoRNP Nucleolus Pseudouridine synthase 

Abbreviations

3AT

3-amino-triazole

BiFC

Bimolecular fluorescence complementation

CB

Cajal bodies

CDD

Conserved domain database

DAPI

4′,6-diamidino-2-phenylindole

DIC

Differential interference contrast

PPT

Phosphinotricine

snRNAs

Small nuclear RNAs

snRNPs

Small nuclear ribonucleoproteins

snoRNAs

Small nucleolar RNAs

snoRNPs

Small nucleolar ribonucleoproteins

TMG-capped-snRNA

tri-methylguanosine-capped snRNA

Ψ

Pseudouridine

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Inna Lermontova
    • 1
  • Veit Schubert
    • 1
  • Frederik Börnke
    • 2
  • Jiri Macas
    • 3
  • Ingo Schubert
    • 1
  1. 1.Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)GaterslebenGermany
  2. 2.Department of BiochemistryFriedrich-Alexander University Erlangen-NürnbergErlangenGermany
  3. 3.Institute of Plant Molecular BiologyCeske BudejoviceCzech Republic

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