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T-DNA tagged knockout mutation of rice OsGSK1, an orthologue of Arabidopsis BIN2, with enhanced tolerance to various abiotic stresses

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Abstract

T-DNA-tagged rice plants were screened under cold- or salt-stress conditions to determine the genes involved in the molecular mechanism for their abiotic-stress response. Line 0-165-65 was identified as a salt-responsive line. The gene responsible for this GUS-positive phenotype was revealed by inverse PCR as OsGSK1 (O ryza s ativa g lycogen s ynthase k inase3-like gene 1), a member of the plant GSK3/SHAGGY-like protein kinase genes and an orthologue of the Arabidopsis b rassinosteroid in sensitive 2 (BIN2), AtSK21. Northern blot analysis showed that OsGSK1 was most highly detected in the developing panicles, suggesting that its expression is developmental stage specific. Knockout (KO) mutants of OsGSK1 showed enhanced tolerance to cold, heat, salt, and drought stresses when compared with non-transgenic segregants (NT). Overexpression of the full-length OsGSK1 led to a stunted growth phenotype similar to the one observed with the gain-of-function BIN/AtSK21 mutant. This suggests that OsGSK1 might be a functional rice orthologue that serves as a negative regulator of brassinosteroid (BR)-signaling. Therefore, we propose that stress-responsive OsGSK1 may have physiological roles in stress signal-transduction pathways and floral developmental processes.

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Acknowledgments

We thank Priscilla Licht for critical proofreading of the manuscript. This work was funded in part by grants from the Biogreen 21 Program, Rural Development Administration and from the National Research Laboratory Program.

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Authors and Affiliations

  1. Department of Life Science, Sogang University, Seoul, 121-742, Korea

    Serry Koh, Sang-Choon Lee, Min-Kyung Kim & Seong-Ryong Kim

  2. Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, 151-747, Korea

    Jun Ho Koh & Sunghwa Choe

  3. National Research Laboratory of Plant Functional Genomics, Department of Life Science, Pohang University of Science and Technology, Pohang, 790-784, Korea

    Sichul Lee & Gynheung An

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Serry Koh and Sang-Choon Lee are co-first authors.

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11103_2007_9213_MOESM1_ESM.tif

Northern blot analysis of SalT under BL treatment. SalT expression level was increased to maximum at 24 h after BL treatment, suggesting SalT is a BL-responsive gene. C, Control; BL, Brassinolide (TIF 550 kb)

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Koh, S., Lee, SC., Kim, MK. et al. T-DNA tagged knockout mutation of rice OsGSK1, an orthologue of Arabidopsis BIN2, with enhanced tolerance to various abiotic stresses. Plant Mol Biol 65, 453–466 (2007). https://doi.org/10.1007/s11103-007-9213-4

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