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Plant Molecular Biology

, Volume 65, Issue 4, pp 453–466 | Cite as

T-DNA tagged knockout mutation of rice OsGSK1, an orthologue of Arabidopsis BIN2, with enhanced tolerance to various abiotic stresses

  • Serry Koh
  • Sang-Choon Lee
  • Min-Kyung Kim
  • Jun Ho Koh
  • Sichul Lee
  • Gynheung An
  • Sunghwa Choe
  • Seong-Ryong Kim
Article

Abstract

T-DNA-tagged rice plants were screened under cold- or salt-stress conditions to determine the genes involved in the molecular mechanism for their abiotic-stress response. Line 0-165-65 was identified as a salt-responsive line. The gene responsible for this GUS-positive phenotype was revealed by inverse PCR as OsGSK1 (O ryza s ativa g lycogen s ynthase k inase3-like gene 1), a member of the plant GSK3/SHAGGY-like protein kinase genes and an orthologue of the Arabidopsis b rassinosteroid in sensitive 2 (BIN2), AtSK21. Northern blot analysis showed that OsGSK1 was most highly detected in the developing panicles, suggesting that its expression is developmental stage specific. Knockout (KO) mutants of OsGSK1 showed enhanced tolerance to cold, heat, salt, and drought stresses when compared with non-transgenic segregants (NT). Overexpression of the full-length OsGSK1 led to a stunted growth phenotype similar to the one observed with the gain-of-function BIN/AtSK21 mutant. This suggests that OsGSK1 might be a functional rice orthologue that serves as a negative regulator of brassinosteroid (BR)-signaling. Therefore, we propose that stress-responsive OsGSK1 may have physiological roles in stress signal-transduction pathways and floral developmental processes.

Keywords

Abiotic stress BR-signaling GUS OsGSK1 T-DNA-tagged rice 

Notes

Acknowledgments

We thank Priscilla Licht for critical proofreading of the manuscript. This work was funded in part by grants from the Biogreen 21 Program, Rural Development Administration and from the National Research Laboratory Program.

Supplementary material

11103_2007_9213_MOESM1_ESM.tif (551 kb)
Northern blot analysis of SalT under BL treatment. SalT expression level was increased to maximum at 24 h after BL treatment, suggesting SalT is a BL-responsive gene. C, Control; BL, Brassinolide (TIF 550 kb)

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Serry Koh
    • 1
  • Sang-Choon Lee
    • 1
  • Min-Kyung Kim
    • 1
  • Jun Ho Koh
    • 2
  • Sichul Lee
    • 3
  • Gynheung An
    • 3
  • Sunghwa Choe
    • 2
  • Seong-Ryong Kim
    • 1
  1. 1.Department of Life ScienceSogang UniversitySeoulKorea
  2. 2.Department of Biological Sciences, College of Natural SciencesSeoul National UniversitySeoulKorea
  3. 3.National Research Laboratory of Plant Functional Genomics, Department of Life SciencePohang University of Science and TechnologyPohangKorea

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