DORNRÖSCHEN-LIKE, an AP2 gene, is necessary for stamen emergence in Arabidopsis
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To identify novel genes in petal and stamen development, a genetic screen was carried out for enhancers of the unusual B class mutant pistillata-5 (pi-5). In pi-5 flowers, second whorl organs develop as sepals rather than petals, but third whorl stamens are normal. One pi-5 enhancer, dornröschen-like-2 (drnl-2), results in third whorl positions developing as filamentous organs. In addition to enhancing the pi-5 phenotype, drnl-2 mutants also exhibit a phenotype in a wild-type PI background. Although stamen primordia are morphologically visible during early stages of flower development, they fail to enlarge in drnl-2 mutants. DRNL, which encodes a single AP2 domain protein, is expressed in a dynamic pattern in the embryo, seedling, and flower. Analysis of both the drnl-2 mutant phenotype and the DRNL expression pattern in flowers suggests that DRNL plays a critical role in stamen emergence in Arabidopsis.
KeywordsAnther AP2/ERF transcription factor Arabidopsis Flower development Stamen PISTILLATA
Auxin response elements
Auxin response factor
Shoot apical meristem
Scanning electron microscope
We thank John Chandler and Wolfgang Werr for sharing the drnl-1 allele prior to publication, and for productive discussions. We also thank Jason Reed for the arf mutant lines, Hong Ma for his opinion of the anther expression pattern in DRNL, Chuck Daghlian for his help with SEM, Eileen Piwarzyk and Stacey King for comments on the manuscript, and the Arabidopsis Biological Resource Center for seed stocks. This work was supported by a grant from the US National Science Foundation (IBN-0516736).
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