DORNRÖSCHEN-LIKE, an AP2 gene, is necessary for stamen emergence in Arabidopsis
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To identify novel genes in petal and stamen development, a genetic screen was carried out for enhancers of the unusual B class mutant pistillata-5 (pi-5). In pi-5 flowers, second whorl organs develop as sepals rather than petals, but third whorl stamens are normal. One pi-5 enhancer, dornröschen-like-2 (drnl-2), results in third whorl positions developing as filamentous organs. In addition to enhancing the pi-5 phenotype, drnl-2 mutants also exhibit a phenotype in a wild-type PI background. Although stamen primordia are morphologically visible during early stages of flower development, they fail to enlarge in drnl-2 mutants. DRNL, which encodes a single AP2 domain protein, is expressed in a dynamic pattern in the embryo, seedling, and flower. Analysis of both the drnl-2 mutant phenotype and the DRNL expression pattern in flowers suggests that DRNL plays a critical role in stamen emergence in Arabidopsis.
KeywordsAnther AP2/ERF transcription factor Arabidopsis Flower development Stamen PISTILLATA
Auxin response elements
Auxin response factor
Shoot apical meristem
Scanning electron microscope
We thank John Chandler and Wolfgang Werr for sharing the drnl-1 allele prior to publication, and for productive discussions. We also thank Jason Reed for the arf mutant lines, Hong Ma for his opinion of the anther expression pattern in DRNL, Chuck Daghlian for his help with SEM, Eileen Piwarzyk and Stacey King for comments on the manuscript, and the Arabidopsis Biological Resource Center for seed stocks. This work was supported by a grant from the US National Science Foundation (IBN-0516736).
- Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR (2003) Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301:653–657PubMedCrossRefGoogle Scholar
- Hill JP, Lord EM (1989) Floral development in Arabidopsis thaliana: a comparison of the wild-type and the homeotic pistillata mutant. Can J Bot 67:2922–2936Google Scholar
- Knopf R, Trebitsh T (2006) The female-specific CS-ACS1G gene of cucumber. A case of gene duplication and recombination between the non-sex-specific 1-aminocyclopropane-1-carboxylate synthase gene and a branched-chain amino acid transaminase gene. Plant Cell Physiol 47:1217–1228PubMedCrossRefGoogle Scholar
- Komaki MK, Okada K, Nishino E, Shimura Y (1988) Isolation and characterization of novel mutants of Arabidopsis thaliana defective in flower development. Development 104:195–203Google Scholar
- Koornneef M, van Elden J, Hanhart CJ, Stam P, Braaksma FJ, Feenstra WJ (1983) Linkage map of Arabidopsis thaliana. J Hered 74:265–272Google Scholar
- Schwarz-Sommer Z, Hue I, Huijser P, Flor PJ, Hansen R, Tetens F, Lönning W-E, Saedler H, Sommer H (1992) Characterization of the Antirrhinum floral homeotic MADS-box gene deficiens: Evidence for DNA binding and autoregulation of its persistent expression throughout flower development. EMBO J 11:251–263PubMedGoogle Scholar
- Ward JM, Smith AM, Shah PK, Galanti SE, Yi H, Demianski AJ, van der Graaff E, Keller B, Neff MM (2006) A new role for the Arabidopsis AP2 transcription factor, LEAFY PETIOLE, in gibberellin-induced germination is revealed by the misexpression of a homologous gene, SOB2/DRN-LIKE. Plant Cell 18:29–39PubMedCrossRefGoogle Scholar