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Plant Molecular Biology

, 65:189 | Cite as

Rapid evolution and complex structural organization in genomic regions harboring multiple prolamin genes in the polyploid wheat genome

  • Shuangcheng Gao
  • Yong Qiang GuEmail author
  • Jiajie Wu
  • Devin Coleman-Derr
  • Naxin Huo
  • Curt Crossman
  • Jizeng Jia
  • Qi Zuo
  • Zhenglong Ren
  • Olin D. Anderson
  • Xiuying KongEmail author
Article

Abstract

Genes encoding wheat prolamins belong to complicated multi-gene families in the wheat genome. To understand the structural complexity of storage protein loci, we sequenced and analyzed orthologous regions containing both gliadin and LMW-glutenin genes from the A and B genomes of a tetraploid wheat species, Triticum turgidum ssp. durum. Despite their physical proximity to one another, the gliadin genes and LMW-glutenin genes are organized quite differently. The gliadin genes are found to be more clustered than the LMW-glutenin genes which are separated from each other by much larger distances. The separation of the LMW-glutenin genes is the result of both the insertion of large blocks of repetitive DNA owing to the rapid amplification of retrotransposons and the presence of genetic loci interspersed between them. Sequence comparisons of the orthologous regions reveal that gene movement could be one of the major factors contributing to the violation of microcolinearity between the homoeologous A and B genomes in wheat. The rapid sequence rearrangements and differential insertion of repetitive DNA has caused the gene islands to be not conserved in compared regions. In addition, we demonstrated that the i-type LMW-glutenin originated from a deletion of 33-bps in the 5′ coding region of the m-type gene. Our results show that multiple rounds of segmental duplication of prolamin genes have driven the amplification of the ω-gliadin genes in the region; such segmental duplication could greatly increase the repetitive DNA content in the genome depending on the amount of repetitive DNA present in the original duplicate region.

Keywords

Prolamin LMW-glutenin Gliadin Genome evolution Gene duplication Transposable element 

Notes

Acknowledgements

We thank Mingcheng Luo for the assistance in BAC fingerprinting and Gerald Lazo for bioinformatics support. This work was partially supported by grants from National Basic Research Program of China (2002CB111301) and National Natural Science Foundation of China (30571158). Work at WRRC is supported by US, Department of Agriculture-Agriculture Research Service Grant CRIS 5325022100-011.

Supplementary material

11103_2007_9208_MOESM1_ESM.pdf (18 kb)
Nested insertion of retroelements and subsequent deletion in the intergenic region between two LMW-glutenin genes. Two LMW-glutenin genes are represented by black boxes. The start position of each LMW-glutenin gene in the contiguous 265-kb region is provided. Retroelements were annotated based on BLAST searches against the TREP database (http://wheat.pw.usda.gov/ITMI/Repeats/). A repetitive DNA with “s” stands for a solo retroelement and “p” for a partial retroelement. Deletion(s) has occurred in the region. The putative deleted portion from each retroelement is indicated with a blue region. The exact sequencelength deleted is difficult to determine. However, It can be expected that without the deletion event, the two LMW-glutenin genes could be separated by a much larger distance. Insertion of Glabra2-like gene into a Fatimah retroelement is indicated. (PDF 19 kb)

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Shuangcheng Gao
    • 1
  • Yong Qiang Gu
    • 2
    Email author
  • Jiajie Wu
    • 1
  • Devin Coleman-Derr
    • 2
  • Naxin Huo
    • 2
  • Curt Crossman
    • 2
  • Jizeng Jia
    • 1
  • Qi Zuo
    • 1
  • Zhenglong Ren
    • 3
  • Olin D. Anderson
    • 2
  • Xiuying Kong
    • 1
    Email author
  1. 1.Key Laboratory of Crop Germplasm & Biotechnology, MOA, Institute of Crop SciencesChinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic ImprovementBeijingP.R. China
  2. 2.United States Department of Agriculture, Agricultural Research ServiceWestern Regional Research CenterAlbanyUSA
  3. 3.State Key Laboratory of Plant Breeding and GeneticsSichuan Agricultural UniversityYa-anP.R. China

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