Analysis of gene-trap Ds rice populations in Korea
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Insertional mutagen-mediated gene tagging populations have been essential resources for analyzing the function of plant genes. In rice, maize transposable elements have been successfully utilized to produce transposant populations. However, many generations and substantial field space are required to obtain a sufficiently sized transposant population. In rice, the japonica and indica subspecies are phenotypically and genetically divergent. Here, callus cultures with seeds carrying Ac and Ds were used to produce 89,700 lines of Dongjin, a japonica cultivar, and 6,200 lines of MGRI079, whose genome is composed of a mixture of the genetic backgrounds of japonica and indica. Of the more than 3,000 lines examined, 67% had Ds elements. Among the Ds-carrying lines, 81% of Dongjin and 63% of MGRI079 contained transposed Ds, with an average of around 2.0 copies. By examining more than 15,000 lines, it was found that 12% expressed the reporter gene GUS during the early-seedling stage. GUS was expressed in root hairs and crown root initials at estimated frequencies of 0.78% and 0.34%, respectively. The 5,271 analyzed Ds loci were found to be randomly distributed over all of the rice chromosomes.
KeywordsRice Ac/Ds Insertional population Gene trap Flanking sequence tags
This project was a collaboration among laboratories at Gyeongsang National University, the NIAB, and YARI. This work was supported by a grant (code # CG151) from Crop Functional Genomics Center of the 21st Century Frontier Research Program, BioGreen 21 Program (Rural Development Administration), by Basic Research Fund (NIAB, 06-2-12-7-2), Korea Research Foundation Grant (KRF-2003-015-C00636), and from the KOSEF/MOST to the Environmental Biotechnology National Core Research Center (R15-2003-012-01001-0). This work was also supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) to Park Sung Han (KRF-2006-351-F00001) and Huang Jin (F00026). Tae Yong Oh and Yuan-hu Xuan are recipients of fellowships of the Brain Korea21 project.
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