Plant Molecular Biology

, 62:825 | Cite as

BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways

  • Nayelli Marsch-Martinez
  • Raffaella Greco
  • Jörg D. Becker
  • Shital Dixit
  • Jan H. W. Bergervoet
  • Aarati Karaba
  • Stefan de Folter
  • Andy Pereira


The BOLITA (BOL) gene, an AP2/ERF transcription factor, was characterized with the help of an activation tag mutant and overexpression lines in Arabidopsis and tobacco. The leaf size of plants overexpressing BOL was smaller than wild type plants due to a reduction in both cell size and cell number. Moreover, severe overexpressors showed ectopic callus formation in roots. Accordingly, global gene expression analysis using the overexpression mutant reflected the alterations in cell proliferation, differentiation and growth through expression changes in RBR, CYCD, and TCP genes, as well as genes involved in cell expansion (i.e. expansins and the actin remodeling factor ADF5). Furthermore, the expression of hormone signaling (i.e. auxin and cytokinin), biosynthesis (i.e. ethylene and jasmonic acid) and regulatory genes was found to be perturbed in bol-D mutant leaves.


AP2/ERF transcription factor Organ size Cell growth Cell proliferation and differentiation Cell cycle CyclinD/retinoblastoma pathway 









Naphthalenacetic acid


Indole-3-acetic acid


1-Aminocyclopropane-1-carboxylic acid




Silver thiosulphate


Small auxin up-regulated RNAs



We thank Robert Sevenier, Sacco de Vries and Cathy Albracht, Kim Boutilier, Asaph Aharoni, and Gert van Arkel for useful suggestions, discussions and help with materials, and Jeroen Peters and Wim Dirkse for technical help with the flow cytometry experiment. We are grateful to Jose Feijo and Margarida Oliveira as organizers of the 2004 EMBO course on Development at Oeiras, Portugal, and Assaf Zemach for collaboration during the global gene expression analysis. Isabelle Bertin is thanked for technical assistance, and Daan Jaspers and Gerrit Stunnenberg for plant care. J.D.B. was supported by fellowship SFRH/BPD/3619/2000 from Fundacão para a Ciência e a Tecnologia. S.D. and A.K. were supported by fellowships from WOTRO-NWO.

Supplementary material

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Nayelli Marsch-Martinez
    • 1
  • Raffaella Greco
    • 1
  • Jörg D. Becker
    • 3
  • Shital Dixit
    • 1
  • Jan H. W. Bergervoet
    • 1
  • Aarati Karaba
    • 1
  • Stefan de Folter
    • 1
  • Andy Pereira
    • 2
  1. 1.Plant Research InternationalWageningen University and Research CentreWageningenThe Netherlands
  2. 2.Virginia Bioinformatics InstituteBlacksburgUSA
  3. 3.Centro de Biologia do DesenvolvimientoInstituto Gulbenkian de CiênciaOeirasPortugal

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