Plant Molecular Biology

, Volume 62, Issue 4–5, pp 561–578 | Cite as

Transcriptional changes in powdery mildew infected wheat and Arabidopsis leaves undergoing syringolin-triggered hypersensitive cell death at infection sites

  • Kathrin Michel
  • Olaf Abderhalden
  • Rémy Bruggmann
  • Robert DudlerEmail author


Blumeria graminis f.sp. tritici, the causal agent of powdery mildew in wheat, is an obligate biotrophic fungus that exclusively invades epidermal cells. As previously shown, spraying of a solution of syringolin A, a circular peptide derivative secreted by the phytopathogenic bacterium Pseudomonas syringae pv. syringae, triggers hypersensitive cell death at infection sites in powdery mildew infected wheat. Thus, the fungus is essentially eradicated. Here we show that syringolin A also triggers hypersensitive cell death in Arabidopsis infected with the powdery mildew fungus Erysiphe cichoracearum. To monitor transcriptional changes associated with this effect, we cloned 307 cDNA clones representing 158 unigenes from powdery mildew infected, syringolin A sprayed wheat leaves by a suppression subtractive hybridization cloning procedure. These cDNAs were microarrayed onto glass slides together with 1088 cDNA-AFLP clones from powdery mildew-infected wheat. Microarray hybridization experiments were performed with probes derived from leaves, epidermal tissue, and mesophyll preparations of mildewed or uninfected wheat plants after syringolin A or control treatment. Similar experiments were performed in Arabidopsis using the Affymetrix ATH1 whole genome GeneChip. The results indicate a conserved mode of action of syringolin A as similar gene groups are induced in both species. Prominent groups include genes associated with the proteasomal degradation pathway, mitochondrial and other heat shock genes, genes involved in mitochondrial alternative electron pathways, and genes encoding glycolytic and fermentative enzymes. Surprisingly, in both species the observed transcriptional response to syringolin A was considerably weaker in infected plants as compared to uninfected plants. The results lead to the working hypothesis that cell death observed at infection sites may result from a parasite-induced suppression of the transcriptional response and thus to insufficient production of protective proteins necessary for the recovery of these cells from whatever insult is imposed by syringolin A.


Blumeria graminis Erysiphe cichoracearum Hypersensitive response Microarray Pseudomonas syringae 


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We thank E.E. Farmer and P. Reymond, University of Lausanne, for use of the Gene Expression Laboratory Microarray facility at the Department of Plant Molecular Biology, U. Wagner and A. Patrignani, Functional Genomics Center Zurich, as well as T. Wicker for help with bioinformatics tasks, and R. Honegger for help with the identification of Erysiphe cichoracearum. Support by the Swiss National Science Foundation (grant 3100A0–100046), the Functional Genomics Center Zurich, and the Foundation for Scientific Research at the University of Zurich is greatfully acknowledged.

Supplementary material


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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Kathrin Michel
    • 1
  • Olaf Abderhalden
    • 1
  • Rémy Bruggmann
    • 1
    • 2
  • Robert Dudler
    • 1
    Email author
  1. 1.Institute of Plant BiologyUniversity of ZurichZurichSwitzerland
  2. 2.Institute for Bioinformatics, GSF Research Center for Environment and HealthMunich Information Center for Protein Sequences (MIPS)NeuherbergGermany

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