Plant Molecular Biology

, Volume 62, Issue 3, pp 351–369 | Cite as

EST database for early flower development in California poppy (Eschscholzia californica Cham., Papaveraceae) tags over 6000 genes from a basal eudicot

  • John E. Carlson
  • James H. Leebens-Mack
  • P. Kerr Wall
  • Laura M. Zahn
  • Lukas A. Mueller
  • Lena L. Landherr
  • Yi Hu
  • Daniel C. Ilut
  • Jennifer M. Arrington
  • Stephanie Choirean
  • Annette Becker
  • Dawn Field
  • Steven D. Tanksley
  • Hong Ma
  • Claude W. dePamphilis
Original Paper

Abstract

The Floral Genome Project (FGP) selected California poppy (Eschscholzia californica Cham. ssp. Californica) to help identify new florally-expressed genes related to floral diversity in basal eudicots. A large, non-normalized cDNA library was constructed from premeiotic and meiotic floral buds and sequenced to generate a database of 9079 high quality Expressed Sequence Tags (ESTs). These sequences clustered into 5713 unigenes, including 1414 contigs and 4299 singletons. Homologs of genes regulating many aspects of flower development were identified, including those for organ identity and development, cell and tissue differentiation, cell cycle control, and secondary metabolism. Over 5% of the transcriptome consisted of homologs to known floral gene families. Most are the first representatives of their respective gene families in basal eudicots and their conservation suggests they are important for floral development and/or function. App. 10% of the transcripts encoded transcription factors and other regulatory genes, including nine genes from the seven major lineages of the important MADS-box family of developmental regulators. Homologs of alkaloid pathway genes were also recovered, providing opportunities to explore adaptive evolution in secondary products. Furthermore, comparison of the poppy ESTs with the Arabidopsis genome provided support for putative Arabidopsis genes that previously lacked annotation. Finally, over 1800 unique sequences had no observable homology in the public databases. The California poppy EST database and library will help bridge our understanding of flower initiation and development among higher eudicot and monocot model plants and provide new opportunities for comparative analysis of gene families across angiosperm species.

Keywords

EST database Flower development California poppy Basal eudicot 

Abbreviations

ABI

Applied Biosystems

AG

AGAMOUS gene

AGL

AGAMOUS-like gene

AP

APETALA gene

DEF

DEFICIENS gene

DEPC

diethylpyrocarbonate

EF-1

Elongation Factor 1-alpha gene

ESca

Eschscholzia californica

EST

Expressed Sequence Tag

FIM

FIMBRIATA gene

FLO

FLORICAULA gene

GO

Gene Ontology Consortium

GLO

GLOBOSA gene

Ks

rate of synonymous substitutions

LFY

LEAFY gene

Mbp

Million base pairs

MYA

Million years ago

MYB

myeloblastosis-like gene

NCBI

National Center for Biotechnology Information

PCR

polymerase chain reaction

PGN

Plant Genome Network

PI

PISTILLATA gene

PLE

PLENA gene

rRNA

ribosomal RNA

RACE

Rapid Amplification of cDNA Ends

RCA

Rolling Circle Amplification

RuBisCO

ribulose-1;5-bisphosphate carboxylase; ssp., species

TAIR

The Arabidopsis Information Resource web site

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Supplementary material

11103_2006_9025_MOESM1_ESM.xls (586 kb)
Supplementary material

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • John E. Carlson
    • 1
  • James H. Leebens-Mack
    • 2
    • 7
  • P. Kerr Wall
    • 2
  • Laura M. Zahn
    • 2
  • Lukas A. Mueller
    • 3
  • Lena L. Landherr
    • 2
  • Yi Hu
    • 2
  • Daniel C. Ilut
    • 3
  • Jennifer M. Arrington
    • 2
    • 4
  • Stephanie Choirean
    • 2
  • Annette Becker
    • 5
  • Dawn Field
    • 6
  • Steven D. Tanksley
    • 3
  • Hong Ma
    • 2
  • Claude W. dePamphilis
    • 2
  1. 1.The School of Forest Resources and Huck Institutes for Life SciencesPennsylvania State UniversityUniversity ParkUSA
  2. 2.Department of Biology, The Huck Institutes of the Life SciencesThe Pennsylvania State UniversityUniversity ParkUSA
  3. 3.Department of Plant BreedingCornell UniversityIthacaUSA
  4. 4.Department of BiologyRandolph-Macon Woman’s CollegeLynchburgUSA
  5. 5.Evolutionary Developmental Genetics GroupUniversity of BremenBremenGermany
  6. 6.Oxford Centre for Ecology and HydrologyOxfordUK
  7. 7.Department of Plant BiologyUniversity of GeorgiaAthensUSA

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