Plant Molecular Biology

, Volume 62, Issue 1–2, pp 247–259 | Cite as

Identification of a Drought Tolerant Introgression Line Derived from Dongxiang Common Wild Rice (O. rufipogon Griff.)

  • Xia Zhang
  • Shaoxia Zhou
  • Yongcai Fu
  • Zhen Su
  • Xiangkun Wang
  • Chuanqing Sun
Original Paper

Abstract

Construction of introgression lines using cultivated rice as recipient and wild rice is a novel approach to explore primitive and broad genetic resources in rice breeding. We recently generated a set of 159 introgression lines via a backcrossing program using an elite Indica cultivar rice Guichao 2 (O. sativa L. ssp. indica) as recipient and a common wild rice Dongxiang accession (O. rufipogon Griff.) as donor. In this study, we have evaluated the previously constructed 159 introgression lines for drought-tolerance. A total of 12 quantitative trait loci (QTLs) related to drought tolerance were mapped. Furthermore, a drought tolerant introgression line, IL23, was identified and characterized. Genotype analysis of IL23 demonstrated that IL23 contained two QTLs associated with drought tolerance, qSDT2-1 and qSDT12-2, which were located on chromosome 2 and 12 within the two introgressed segments derived from the common wild rice, respectively. Physiological characterization, including measurement of water loss, osmotic potential, electrolytical leakage, MDA content, soluble sugars content and the leaf temperature, revealed that IL23 showed the characteristics associated with drought tolerance. Identification and characterization of IL23 would provide a useful basis for isolation of novel genes associated with drought tolerance and for molecular breeding of drought tolerant rice. Furthermore, the results in this study indicated that construction of introgression lines from common wild rice should be an appropriate approach to obtain favorable genetic materials.

Keywords

Common wild rice (O. rufipogon Griff.) QTL Introgression lines Polyethylene glycol (PEG) Drought tolerance 

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References

  1. Cabuslay G, Ito O, Alejar A (1999) In: O’Toole J, Ito O, Hardy B (eds) Genetic improvement of rice for water-limited environments. International Rice research Institute, Manila, pp 99–116Google Scholar
  2. Champoux MC, Wang G, Sarkaruag S, Mackill DJ, O’Toole JC, Huang N, McCouch SR (1995) Locating genes associated with root morphology and drought avoidance in rice via linkage to molecular markers. Theor Appl Genet 90:969–981CrossRefGoogle Scholar
  3. Chen ZZ, Hong XH, Zhang HR, Wang YQ, Li X, Zhu JK, Gong ZZ (2005) Disruption of the cellulose synthase gene, AtCesA8/IRX1, enhances drought and osmotic stress tolerance in Arabidopsis. Plant J 43:273–283PubMedMATHCrossRefGoogle Scholar
  4. Crasta OR, Xu WW, Rosenow DT, Mullet J, Nguyen HT (1999) Mapping of post-flowering drought resistance traits in grain sorghum: association between QTLs influencing premature senescence and maturity. Mol Gen Genet 226:579–588Google Scholar
  5. Dey MM, Upadhaya HK (1996) Yield loss due to drought, cold, and submergence in Asia. In: Evenson RE, Herdt RW, Hossain M (eds) Rice research in Asia, progress and priorities. Oxford University Press, Cary NC, pp 231–242Google Scholar
  6. Eshed Y, Zamir D (1994) Introgressions from Lycopersicon pennellii can improve the soluble-solids yield of tomato hybrids. Therot Appl Genet 88:891–897CrossRefGoogle Scholar
  7. Frary A, Nesbitt TC, Grandillo S, Knaap E, Cong B, Liu J, Meller J, Elber R, Alpert KB, Tanksley SD (2000) fw2.2: a quantitative trait locus key to the evolution of tomato fruit size. Science 289(5476):71–72CrossRefGoogle Scholar
  8. Fridman E, Carrari F, Liu YS, Fernie AR, Zamir D (2004) Zooming in on a quantitative trait for tomato yield using interspecific introgressions. Science 305(5691):1786–1789PubMedCrossRefADSGoogle Scholar
  9. Frova C, Krajewski P, di Fonzo N, Villa M, Sari-Goria M (1999) Genetic analysis of drought tolerance in maize by molecular markers. I. Yield components. Theor Appl Genet 99:280–288CrossRefGoogle Scholar
  10. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. Arch Biochem Biophys 125:189–198PubMedCrossRefGoogle Scholar
  11. Inan G, Zhang Q, Li PH, Wang ZL, Cao ZY, Zhang H, Zhang CQ, Quist TM, Goodwin SM, Zhu JL, Shi HZ, Damsz B, Charbaji T, Gong QQ, Ma SS, Fredricksen M, Galbraith DW, Jenks MA, Rhodes D, Hasegawa PM, Bohnert HJ, Joly RJ, Bressan RA, Zhu JK (2004) Salt cress. A halophyte and cryophyte Arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles. Plant Physiol 125:1718–1737CrossRefGoogle Scholar
  12. Jung YC, Lee HJ, Yum SS, Soh WY, Cho DY, Auh CK, Lee TK, Soh HC, Kim YS, Lee SC (2005) Drought-inducible-but ABA-independent-thaumatin-like protein from carrot (Daucus carota L.). Plant Cell Rep 24(6):366–373PubMedCrossRefGoogle Scholar
  13. Kreps JA, Wu Y, Chang HS, Zhu T, Wang X, Harper JF (2002) Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol 130(4):2129–2141PubMedCrossRefGoogle Scholar
  14. Kunert KJ, Ederer M (1985) Leaf aging and lipid peroxidation: the role of antioxidants vitamin C and E. Plant Physiol 65:85–88CrossRefGoogle Scholar
  15. Lanceras JC, Pantuwan G, Jongdee B, Toojinda T (2004) Quantitative trait loci associated with drought tolerance at reproductive stage in rice. Plant Physiol 135:384–399PubMedCrossRefGoogle Scholar
  16. Lilley JM, Ludlow MM, McCouch SR, Otoole JC (1996) Locating QTL for osmotic adjustment and dehydration tolerance in rice. J Exp Bot 47:1427–1436Google Scholar
  17. Lu Z, Neumann PM (1999) Water stress inhibits hydraulic conductance and leaf growth in rice seedlings but not the transport of water via mercury-sensitive water channels in the root. Plant Physiol 120(1):143–152PubMedCrossRefGoogle Scholar
  18. Manly KF, Cudmore RH Jr, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932PubMedCrossRefGoogle Scholar
  19. Morgan JM (1977) Differences in osmoregulation between wheat genotype. Nature 270(5634):234–235CrossRefGoogle Scholar
  20. Oka HI (1988) Origin of cultivated rice. Developments in crop science, vol 14. Elsevier Science, AmsterdamGoogle Scholar
  21. Ozturk ZN, Talamé V, Deyholos M, Michalowski CB, Galbraith DW, Gozukirmizi N, Tuberosa R, Bohnert HJ (2002) Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley. Plant Mol Biol 48:551–573CrossRefGoogle Scholar
  22. Panaud O, Chen X, McCouch SR (1996) Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Gent 252:597–607Google Scholar
  23. Price AH, Tomos AD (1997) Genetic dissection of root growth in rice (Oryza sativa L.): mapping quantitative trait loci using molecular markers. Theor Appl Genet 95:143–152CrossRefGoogle Scholar
  24. Price AH, Townend J, Jones MP, Audebert A, Courtois B (2002) Mapping QTL associated with drought avoidance in upland rice grown in the Philippines and West Africa. Plant Mol Biol 48:683–695PubMedCrossRefGoogle Scholar
  25. Rogers OS, Bendich AJ (1988) Extraction of DNA from plant tissue. Plant Mol Biol Manual A6:1–10Google Scholar
  26. Second G (1982) Origin of the genetic diversity of cultivated rice (Oryza spp.), study of the polymorphism scored at 40 isozyme loci. Jpn J Genet 57:25–57Google Scholar
  27. Smirnoff N (1998) Plant resistance to environmental stress. Curr Opin Biotechnol 9(2):214–219PubMedCrossRefGoogle Scholar
  28. Sun CQ, Wang XK, Yoshimura A, Iwata N (2001) Comparison of the genetic diversity of common wild rice (Oryza rufipogon Griff.) and cultivated rice (O. sativa L.) using RFLP markers. Theor Appl Genet 102:157–162CrossRefGoogle Scholar
  29. Temnykh S, Park WD, Ayres N, Cartinhour S, Hauck N, Lipovich L, Cho YG, Ishii T, McCouch S (2000) Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theor Appl Genet 100:697–712CrossRefGoogle Scholar
  30. Tian F, Li DJ, Fu Q, Zhu ZF, Fu YC, Wang XK, Sun CQ (2005) Construction of introgression lines carrying wild rice (Oryza rufipogon Griff.) segments in cultivated rice (O. sativa L.) background and characterization of introgressed segments associated with yield-related traits. Theor Appl Genet. Published online (Dec 6, 2005)Google Scholar
  31. Walia H, Wilson C, Condamine P, Liu X, Ismail AM, Zeng LH, Wanamaker SI, Mandal J, Xu J, Cui XP, Close TJ (2005) Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage. Plant Physiol 139:822–835PubMedCrossRefGoogle Scholar
  32. Wang ZY, Second G, Tanksley SD (1992) Polymorphism and phylogenetic relationships among species in the genus Oryza as determined by analysis of nuclear RFLPs. Thero Appl Genet 83:565–581Google Scholar
  33. Wing RA, Ammiraju JSS, Luo M, Kim HR, Yu Y, Kudrna D, Goicoechea JL, Wang W, Nelson W, Rao K, Brar D, Mackill DJ, Han B, Soderlund C, Stein L, SanMiguel P, Jackson S (2005) The Oryza map alignment project:the golden path to unlocking the genetic potential of wild rice species. Plant Mol Biol 59:53–62PubMedCrossRefGoogle Scholar
  34. Yue B, Xiong LZ, Xue WY, Xing YZ, Luo LJ, Xu CG (2005) Genetic analysis for drought resistance of rice at reproductive stage in field with different types of soil. Theor Appl Genet 111:1127–1136PubMedCrossRefGoogle Scholar
  35. Zhang X, Guo SL, Yin HB, Xiong DJ, Zhang H, Zhao YX (2004) Molecular cloning and identification of a heat shock cognate protein 70 gene, Thhsc70, in Thellungiella halophila. Acta Botanica Sinica 46(10):1212–1219Google Scholar
  36. Zheng J, Zhao J, Tao Y, Wang J, Liu Y, Fu J, Jin Y, Gao P, Zhang J, Bai Y, Wang G (2004) Isolation and analysis of water stress induced genes in maize seedlings by substractive PCR and cDNA macroarray. Plant Mol Biol 55:807–823PubMedGoogle Scholar
  37. Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Xia Zhang
    • 1
    • 2
    • 3
  • Shaoxia Zhou
    • 1
    • 2
    • 3
  • Yongcai Fu
    • 1
    • 2
    • 3
  • Zhen Su
    • 4
  • Xiangkun Wang
    • 1
    • 2
    • 3
  • Chuanqing Sun
    • 1
    • 2
    • 3
  1. 1.Department of Plant Genetic and Breeding and State Key Laboratory of AgrobiotechnologyChina Agricultural UniversityBeijingChina
  2. 2.Beijing Key Laboratory of Crop Genetic ImprovementBeijingChina
  3. 3.Key Laboratory of Crop Genetic Improvement and Genome of Ministry of AgricultureBeijingChina
  4. 4.State Key Laboratory of Plant Physiology and Biochemistry, College of Biological SciencesChina Agricultural UniversityBeijingChina

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