Plant Molecular Biology

, Volume 62, Issue 1–2, pp 29–42

Loss of NECROTIC SPOTTED LESIONS 1 associates with cell death and defense responses in Arabidopsis thaliana

  • Yoshiteru Noutoshi
  • Takashi Kuromori
  • Takuji Wada
  • Takashi Hirayama
  • Asako Kamiya
  • Yuko Imura
  • Michiko Yasuda
  • Hideo Nakashita
  • Ken Shirasu
  • Kazuo Shinozaki
Article

Abstract

We isolated a lesion mimic mutant, necrotic spotted lesions 1 (nsl1), from Ds-tagged Arabidopsis thaliana accession No-0. The nsl1 mutant exhibits a growth retardation phenotype and develops spotted necrotic lesions on its rosette and cauline leaves. These phenotypes occur in the absence of pathogens indicating that nsl1 mutants may constitutively express defense responses. Consistent with this idea, nsl1 accumulates high levels of callose and autofluorescent phenolic compounds localized to the necrotic lesions. Furthermore RNA gel blot analysis revealed that genes associated with disease resistance activation are upregulated in the nsl1 mutants and these plants contain elevated levels of salicylic acid (SA). Crossing nsl1 with an SA deficient mutant, eds16-1, revealed that the nsl1 lesions and growth retardation are dependent upon SA. The nsl1 phenotypes are not suppressed under either the rar1-10 or sgt1b-1 genetic background. NSL1 encodes a novel 612aa protein which contains a membrane-attack complex/perforin (MACPF) domain, which is conserved in bacteria, fungi, mammals and plants. The possible modes of action of NSL1 protein in negative regulation of cell death programs and defense responses are discussed.

Keywords

Cell death Defense responses Salicylic acid MACPF domain 

Abbreviations

Avr

avirulence

CNGC

cyclic nucleotide-gated cation channel

Ds

Dissociation transposon

H2O2

hydrogen peroxide

HR

hypersensitive response

LRR

leucine rich repeat

MACPF

membrane attack complex and perforin

NahG

salicylate hydroxylase

MAP kinase

mitogen-activated protein kinase

NBS

nucleotide binding site

PCD

programmed cell death

PCR

polymerase chain reaction

PR genes

pathogenesis-related genes

R gene

disease resistance gene

ROS

reactive oxygen species

SA

salicylic acid

SAR

systemic acquired resistance

TIR

Toll/interleukin-1 receptor

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Yoshiteru Noutoshi
    • 1
    • 7
  • Takashi Kuromori
    • 2
  • Takuji Wada
    • 3
  • Takashi Hirayama
    • 2
    • 4
  • Asako Kamiya
    • 2
  • Yuko Imura
    • 2
  • Michiko Yasuda
    • 5
  • Hideo Nakashita
    • 5
  • Ken Shirasu
    • 3
    • 6
  • Kazuo Shinozaki
    • 1
    • 2
    • 3
  1. 1.Plant Molecular BiologyRIKEN Tsukuba InstituteIbarakiJapan
  2. 2.Genomic Sciences CenterRIKEN Yokohama InstituteYokohamaJapan
  3. 3.Plant Science CenterRIKEN Yokohama InstituteYokohamaJapan
  4. 4.International Graduate School of Arts and ScienceYokohama City Univ.YokohamaJapan
  5. 5.Plant Functions LaboratoryRIKEN InstituteSaitamaJapan
  6. 6.The Sainsbury LaboratoryJohn Innes CentreNorwichUK
  7. 7.The Sainsbury LaboratoryJohn Innes CentreNorwichUK

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