Plant Molecular Biology

, Volume 58, Issue 6, pp 789–807

Alfalfa Mob1-like Genes are Expressed in Reproductive Organs during Meiosis and Gametogenesis

  • Sandra Citterio
  • Emidio Albertini
  • Serena Varotto
  • Erika Feltrin
  • Marica Soattin
  • Gianpiero Marconi
  • Sergio Sgorbati
  • Margherita Lucchin
  • Gianni Barcaccia
Article

Abstract

Mps-one-binder (Mob) proteins play an important role in chromosome separation and cell plate formation in yeast. We cloned two Mob1-like genes from alfalfa (Medicago sativa L.) and show that one gene is constitutively expressed while the other is expressed only in flower buds during sporogenesis and gametogenesis. For the analysis of gene expression during reproduction in alfalfa wild-types and apomeiotic mutants, a specific antisense riboprobe was designed for MsMob1 transcripts and a polyclonal antibody was raised against MsMob1 proteins. In situ mRNA localization as well as protein immunolocalization proved that MsMob1-like genes are specifically expressed in degenerating megaspores of normal ovules and in enlarged megaspore mother cells and embryo sacs of apomeiotic ovules. Gene products were also found in microspore tetrads at the beginning of pollen development as well as in tapetum cells of anthers undergoing programmed cell death to allow pollen dispersal at maturity. Overall results suggest that MsMob1-like genes can play a key role during the reproductive pathway in plants.

Key words

Apomeiosis gametogenesis Medicago sativa L. Mps-one-binder programmed cell death reproductive mutants 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Supplementary material

supp.pdf (38 kb)
Supplementary material

References

  1. Albertini, E., Marconi, G., Barcaccia, G., Raggi, L., Falcinelli, M. 2004Isolation of candidate genes for apomixis in Poa pratensis LPlant Mol. Biol.56879894CrossRefPubMedGoogle Scholar
  2. Allen, R.T., Cluck, M.W., Agrawal, D.K. 1998Mechanisms controlling cellular suicide: role of Bc11 and caspasesCell Mol. Life Sci.54427445CrossRefPubMedGoogle Scholar
  3. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J. 1990Basic local alignments search toolJ. Mol. Biol.215403410CrossRefPubMedGoogle Scholar
  4. Barcaccia, G., Tavoletti, S., Falcinelli, M., Veronesi, F. 1997aEnvironmental influence on the frequency and viability of meiotic and apomeiotic cells in a diploid mutant of alfalfaCrop Sci.377076Google Scholar
  5. Barcaccia, G., Tavoletti, S., Falcinelli, M., Veronesi, F. 1997bVerification of the parthenogenetic capability of unreduced eggs in an alfalfa mutant by a progeny test based on morphological and molecular markersPlant Breed.116475479Google Scholar
  6. Barcaccia, G., Albertini, E., Tavoletti, S, Falcinelli, M., Veronesi, F. 1999AFLP fingerprinting in Medicago spp.: its development and application in linkage mappingPlant Breed.118335340CrossRefGoogle Scholar
  7. Barcaccia, G., Albertini, E., Rosellini, D., Tavoletti, S., Veronesi, F. 2000Inheritance and mapping of 2n egg production in diploid alfalfaGenome43528537CrossRefPubMedGoogle Scholar
  8. Barcaccia, G., Varotto, S., Meneghetti, S., Albertini, E., Porceddu, A., Parrini, P., Lucchin, M. 2001Analysis of gene expression during flowering in apomeiotic mutants of Medicago spp.: cloning of ESTs and candidate genes for 2n eggsSex Plant Reprod.14233238CrossRefGoogle Scholar
  9. Barcaccia, G., Tavoletti, S., Mariani, A., Veronesi, F. 2003Occurrence, inheritance and use of reproductive mutants of alfalfa (Medicago spp)Euphytica1333756CrossRefGoogle Scholar
  10. Baskin, T.I. 2000

    The cytoskeleton

    Buchanan, B.Gruissem, W.Jones, R. eds. Biochemistry and Molecular Biology of PlantsAm. Soc. Plant Physiol.USA202258
    Google Scholar
  11. Bedinger, P. 1992The remarkable biology of pollenPlant Cell4879887CrossRefPubMedGoogle Scholar
  12. Citterio S., Varotto, S., Albertini, E., Feltrin, E., Soattin, M., Marconi, G., Sgorbati, S., Lucchin, M. and Barcaccia, G. 2005. Alfalfa Mob1-like proteins are involved in cell proliferation and localize to the division plane during cytokinesis. Proc. of Plant and Animal Genome XIII Conf. San Diego, CA (USA), p. 261.Google Scholar
  13. Devroe, E., Erdjument-Bromage, H., Tempst, P., Silver, P.A. 2004Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinasesJ. Biol. Chem.2792444424451CrossRefPubMedGoogle Scholar
  14. Genualdo, G., Errico, A., Tizzi, A., Conicella, C. 1998 α-tubulin and F-actin distribution during microsporogenesis in a 2n pollen producer of SolanumGenome41636641CrossRefGoogle Scholar
  15. Hammarton, T.C., Lillico, S.G., Welburn, S.C., Mottram, J.C. 2005Trypanosoma brucei Mob1 is required for accurate and efficient cytokinesis but not for exit from mitosisMol. Microbiol.56104116CrossRefPubMedGoogle Scholar
  16. Higgins, D.G., Bleasby, A.J., Fuchs, R. 1992CLUSTAL: a package for performing multiple sequence alignments on a micro-computerComput. Appl. Biosci.8189191PubMedGoogle Scholar
  17. Koltunow, A.M. 1993Apomixis: embryo sacs and embryos formed without meiosis and fertilization in ovulesPlant Cell514251437CrossRefPubMedGoogle Scholar
  18. Liu, J.Q., Leggewie, G., Varotto, S., Thompson, R.D. 1999Characterization of an anther-expressed protein kinase gene in the potato Solanum berthaultii and its antisense inhibition in transgenic plantsSex Plant Reprod.11336346CrossRefGoogle Scholar
  19. Luca, F.C., Winey, M. 1998MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidyMol. Biol. Cell.92946PubMedGoogle Scholar
  20. Luca, F.C., Mody, M., Kurischko, C., Roof, D.M., Giddings, T.H., Winey, M. 2001Saccharomyces cerevisiae Mob1p is required for cytokinesis and mitotic exitMol. Cell. Biol.2069726983CrossRefGoogle Scholar
  21. Nogler, G.A. 1984

    Gametophytic apomixis

    Johri, B.M. eds. Embryology of AngiospermsSpringer-VerlagBerlin, Germany475518
    Google Scholar
  22. Onelli, E., Citterio, S., O’Connor, J.E., Levi, M., Sgorbati, S. 1997Flow cytometry, sorting and immunocharacterization with proliferating cell nuclear antigen of cycling and non-cycling cells in synchronized pea root tipsPlanta202188195CrossRefPubMedGoogle Scholar
  23. Pennel, R.I., Lamb, C. 1997Programmed cell death in plantsPlant Cell911571168CrossRefPubMedGoogle Scholar
  24. Pfeiffer, T.W., Bingham, E.T. 1983Abnormal meiosis in alfalfa, Medicagosativa: cytology of 2n eggs and 4n pollen formationCan J. Genet. Cytol.25107112Google Scholar
  25. Ponchon, L., Dumas, C., Kajava, A.V., Fesquet, D., Padilla, A. 2004NMR solution structure of Mob1, a mitotic exit network protein and its interaction with an NDR kinase peptideJ. Mol. Biol.337167182CrossRefPubMedGoogle Scholar
  26. Sambrook, J., Fritsch, E.F., Maniatis, T. 1989Molecular Cloning: A Laboratory Manual, 2nd ednCold Spring Harbor Laboratory PressCold Spring Harbor, New YorkGoogle Scholar
  27. Savidan, Y. 2000

    Apomixis: Genetics and breeding

    Janick, J. eds. Plant Breeding ReviewsWiley and Sons, IncNew York1386
    Google Scholar
  28. Shahmuradov, A., Solovyev, V.V., Gammerman, A.J. 2005Plant promoter prediction with confidence estimationNucleic Acid Res.3310691076CrossRefPubMedGoogle Scholar
  29. Staiger, C.J., Cande, W.Z. 1993

    Cytoskeletal analysis of maize meiotic mutants

    Ormrod, J.C.Francis, D. eds. Molecular and cell biology of the plant cell cycleKluwerDordrecht157171
    Google Scholar
  30. Stavridi, E.S., Harris, K.G., Huyen, Y., Bothos, J., Verwoerd, P.M., Stayrook, S.E., Pavletich, N.P., Jeffrey, P.D., Luca, F.C. 2003Crystal structure of a human Mob1 protein: toward understanding Mob-regulated cell cycle pathwaysStructure1111631170CrossRefPubMedGoogle Scholar
  31. Stegmeier, F., Visintin, R., Amon, A. 2002Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphaseCell108207220CrossRefPubMedGoogle Scholar
  32. Tavoletti, S. 1994Cytological mechanisms of 2n egg formation in a diploid genotype of Medicago sativa subsp. falcataEuphytica7518CrossRefGoogle Scholar
  33. Tavoletti, S., Bingham, E.T., Yandell B.S., Veronesi, F., Osborn, T.C., 1996. Half tetrad analysis in alfalfa using multiple restriction fragment length polymorphism markers. Proc. Natl. Acad. Sci. USA 93: 10918–10922.Google Scholar
  34. Damme, D., Bouget, F.Y., Poucke, K, Inzé, D., Geelen, D. 2004Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteinsPlant J.40386398CrossRefPubMedGoogle Scholar
  35. Biezen, E.A., Jones, J.D.G. 1998The NB-ARC domain: a novel signaling motif shared by plant resistance gene products and regulators of cell death in animalsCurr. Biol.8226227CrossRefGoogle Scholar
  36. Vaux, D.L., Korsmeyer, S.J. 1999Cell death in developmentCell96245254CrossRefPubMedGoogle Scholar
  37. Villemur, R., Haas, N.A., Joyce, C.M., Snustad, D.P., Silflow, C.D. 1994Characterization of four new beta-tubulin genes and their expression during male flower development in maize (Zea mays L.)Plant Mol. Biol.24295315CrossRefPubMedGoogle Scholar
  38. Wu, H., Cheung, A.Y. 2000Programmed cell death in plant reproductionPlant Mol. Biol.44267281CrossRefPubMedGoogle Scholar
  39. Wyllie, A.H. 1980Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activityNature284555556CrossRefPubMedGoogle Scholar
  40. Zhi-Chun Lai Wei, X., Shimizu, T., Ramos, E., Rohrbaugh, R., Nikolaidis, N., Li-Lun Ho, , Ying Li,  2005Control of cell proliferation and apoptosis by Mob1 as tumor suppressor, MatsCell120675685CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Sandra Citterio
    • 1
  • Emidio Albertini
    • 2
  • Serena Varotto
    • 3
  • Erika Feltrin
    • 3
  • Marica Soattin
    • 3
  • Gianpiero Marconi
    • 2
  • Sergio Sgorbati
    • 1
  • Margherita Lucchin
    • 3
  • Gianni Barcaccia
    • 3
  1. 1.Dipartimento di Scienze dell’Ambiente e del TerritorioUniversity of Milano – BicoccaMilanoItaly
  2. 2.Dipartimento di Biologia Vegetale e Biotecnologie AgroambientaliUniversity of PerugiaPerugiaItaly
  3. 3.Laboratory of Plant Genetics and Genomics, Dipartimento di Agronomia Ambientale e Produzioni VegetaliUniversity of Padova-AgripolisLegnaro PadovaItaly

Personalised recommendations