Plant Molecular Biology

, Volume 57, Issue 6, pp 819–833 | Cite as

Arabidopsis Rad51B is important for double-strand DNA breaks repair in somatic cells

  • Keishi Osakabe
  • Kiyomi Abe
  • Hiroaki Yamanouchi
  • Toshio Takyuu
  • Terutaka Yoshioka
  • Yuji Ito
  • Tomohiko Kato
  • Satoshi Tabata
  • Shunsuke Kurei
  • Yasushi Yoshioka
  • Yasunori Machida
  • Motoaki Seki
  • Masatomo Kobayashi
  • Kazuo Shinozaki
  • Hiroaki Ichikawa
  • Seiichi Toki
Article

Abstract

Rad51 paralogs belong to the Rad52 epistasis group of proteins and are involved in homologous recombination (HR), especially the assembly and stabilization of Rad51, which is a homolog of RecA in eukaryotes. We previously cloned and characterized two RAD51 paralogous genes in Arabidopsis, named AtRAD51C and AtXRCC3, which are considered the counterparts of human RAD51C and XRCC3, respectively. Here we describe the identification of RAD51B homologue in Arabidopsis, AtRAD51B. We found a higher expression of AtRAD51B in flower buds and roots. Expression of AtRAD51B was induced by genotoxic stresses such as ionizing irradiation and treatment with a cross-linking reagent, cisplatin. Yeast two-hybrid analysis showed that AtRad51B interacted with AtRad51C. We also found and characterized T-DNA insertion mutant lines. The mutant lines were devoid of AtRAD51B expression, viable and fertile. The mutants were moderately sensitive to γ-ray and hypersensitive to cisplatin. Our results suggest that AtRAD51B gene product is involved in the repair of double-strand DNA breaks (DSBs) via HR

Keywords

Arabidopsis cisplatin γ-ray homologous recombination Rad51 paralogs T-DNA tagging 

Abbreviations

RACE

rapid amplification of cDNA ends

RT-PCR

reverse transcriptase polymerase chain reaction

X-gal

5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside

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Copyright information

© Springer 2005

Authors and Affiliations

  • Keishi Osakabe
    • 1
  • Kiyomi Abe
    • 1
  • Hiroaki Yamanouchi
    • 2
  • Toshio Takyuu
    • 2
  • Terutaka Yoshioka
    • 2
  • Yuji Ito
    • 2
  • Tomohiko Kato
    • 3
  • Satoshi Tabata
    • 3
  • Shunsuke Kurei
    • 4
  • Yasushi Yoshioka
    • 4
  • Yasunori Machida
    • 4
  • Motoaki Seki
    • 5
  • Masatomo Kobayashi
    • 6
  • Kazuo Shinozaki
    • 5
  • Hiroaki Ichikawa
    • 1
  • Seiichi Toki
    • 1
  1. 1.Department of Plant BiotechnologyNational Institute of Agrobiological SciencesTsukubaJapan
  2. 2.Institute of Radiation BreedingNational Institute of Agrobiological SciencesHitachiohmiyaJapan
  3. 3.Kazusa DNA Research InstituteKisarazuJapan
  4. 4.Division of Biological Science, Graduate School of ScienceNagoya UniversityFuro-cho, Chikusa-kuJapan
  5. 5.RIKEN Genomic Sciences Center (GSC)RIKEN Yokohama InstituteTsurumi-kuJapan
  6. 6.Experimental Plant DivisionRIKEN BioResource CenterTsukubaJapan

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