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Plant Molecular Biology

, Volume 59, Issue 6, pp 839–851 | Cite as

The Genomic Organization of Retrotransposons in Brassica oleracea

  • Karine Alix
  • Carol D. Ryder
  • Jay Moore
  • Graham J. King
  • J. S. (Pat) Heslop-HarrisonEmail author
Article

Abstract

We have investigated the copy numbers and genomic organization of five representative reverse transcriptase domains from retrotransposons in Brassica oleracea. Two non-homologous Pseudoviridae (Ty1/copia-like) elements, two Metaviridae (Ty3/gypsy-like) elements (one related to the Athila family) and one Retroposinae (LINE) element were hybridized to a gridded BAC library, “BoB”. The results indicated that the individual LTR retrotransposons (copia and gypsy-like) were represented by between 90 and 320 copies in the haploid genome, with only evidence of a single location for the LINE. Sequence analysis of the same elements against genome survey sequence gave estimates of between 60 and 570, but no LINE was found. There was minimal evidence for clustering between any of these retroelements: only half the randomly expected number of BACs hybridized to both LTR-retrotransposon families. Fluorescent in situ hybridization showed that each of the retroelements had a characteristic genomic distribution. Our results suggest there are preferential sites and perhaps control mechanisms for the insertion or excision of different retrotransposon groups.

Keywords

BAC library copia fluorescent in situ hybridization (FISH) gypsy LINE reverse transcriptase 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Karine Alix
    • 1
    • 2
  • Carol D. Ryder
    • 3
  • Jay Moore
    • 3
  • Graham J. King
    • 4
  • J. S. (Pat) Heslop-Harrison
    • 1
    Email author
  1. 1.Department of BiologyUniversity of LeicesterLeicesterUK
  2. 2.UMR de Génétique Végétale INRA/UPS/CNRS/INA P-G, Ferme du MoulonGif sur YvetteFrance
  3. 3.Warwick HRIWarwickUK
  4. 4.Rothamsted ResearchHarpendenHertfordshireUK

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