Plant Molecular Biology

, Volume 59, Issue 5, pp 713–722 | Cite as

ZmMPK6, a Novel Maize MAP Kinase that Interacts with 14-3-3 Proteins

  • Marco Lalle
  • Sabina Visconti
  • Mauro Marra
  • Lorenzo Camoni
  • Riccardo Velasco
  • Patrizia Aducci
Article

Abstract

Although an increasing body of evidence indicates that plant MAP kinases are involved in a number of cellular processes, such as cell cycle regulation and cellular response to abiotic stresses, hormones and pathogen attack, very little is known about their biochemical properties and regulation mechanism. In this paper we report on the identification and characterization of a novel member of the MAP kinase family from maize, ZmMPK6. The amino acid sequence reveals a high degree of identity with group D plant MAP kinases. Recombinant ZmMPK6, expressed in Escherichia coli, is an active enzyme able to autophosphorylate. Remarkably, ZmMPK6 interacts in vitro with GF14-6, a maize 14-3-3 protein and the interaction is dependent on autophosphorylation. The interacting domain of ZmMPK6 is on the C-terminus and is comprised between amino acid 337 and amino acid 467. Our results represent the first evidence of an interaction between a plant MAP kinase and a 14-3-3 protein. Possible functional roles of this association in vivo are discussed.

Keywords

14-3-3 protein MAP kinase protein-protein interaction Zea mays 

Abbreviations

ERK

Extracellular-signal Regulated Kinase

GST

glutathione S-transferase

MAPK

mitogen-activated protein kinase

MAPKKK

mitogen-activated protein kinase kinase kinase

MEK

Mitogen-activated protein kinase/Extracellular signal-regulated kinase Kinase)

MHA2

Maize H+-ATPase isoform 2

PAGE

polyacrylamide gel electrophoresis

PVDF

polyvinylidene fluoride

RT-PCR

reverse transcriptase polimerase chain reaction

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Copyright information

© Springer 2005

Authors and Affiliations

  • Marco Lalle
    • 1
    • 2
  • Sabina Visconti
    • 1
  • Mauro Marra
    • 3
  • Lorenzo Camoni
    • 1
  • Riccardo Velasco
    • 4
    • 5
  • Patrizia Aducci
    • 1
  1. 1.Department of BiologyUniversity of Rome “Tor Vergata”RomeItaly
  2. 2.Department of Infectious, Parasitic and Immuno-mediated DiseasesIstituto Superiore di Sanita’RomeItaly
  3. 3.Department of Biological and Environmental StudiesUniversity of SannioBeneventoItaly
  4. 4.Max Planck Institut fuer ZuechtungsforschungKoelnGermany
  5. 5.Istituto Agrario San Michele all’AdigeSan Michele a/AdigeItaly

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