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Plant Molecular Biology

, Volume 59, Issue 1, pp 85–97 | Cite as

Chemical- and Irradiation-induced Mutants of Indica Rice IR64 for Forward and Reverse Genetics

  • Jian-Li Wu
  • Chanjian Wu
  • Cailin Lei
  • Marietta Baraoidan
  • Alicia Bordeos
  • Ma. Reina Suzette Madamba
  • Marilou Ramos-Pamplona
  • Ramil Mauleon
  • Arlett Portugal
  • Victor Jun Ulat
  • Richard Bruskiewich
  • Guoliang Wang
  • Jan Leach
  • Gurdev Khush
  • Hei LeungEmail author
Article

Abstract

IR64, the most widely grown indicarice in South and Southeast Asia, possesses many positive agronomic characteristics (e.g., wide adaptability, high yield potential, tolerance to multiple diseases and pests, and good eating quality,) that make it an ideal genotype for identifying mutational changes in traits of agronomic importance. We have produced a large collection of chemical and irradiation-induced IR64 mutants with different genetic lesions that are amenable to both forward and reverse genetics. About 60,000 IR64 mutants have been generated by mutagenesis using chemicals (diepoxybutane and ethylmethanesulfonate) and irradiation (fast neutron and gamma ray). More than 38,000 independent lines have been advanced to M4 generation enabling evaluation of quantitative traits by replicated trials. Morphological variations at vegetative and reproductive stages, including plant architecture, growth habit, pigmentation and various physiological characters, are commonly observed in the four mutagenized populations. Conditional mutants such as gain or loss of resistance to blast, bacterial blight, and tungro disease have been identified at frequencies ranging from 0.01% to 0.1%. Results from pilot experiments indicate that the mutant collections are suitable for reverse genetics through PCR-detection of deletions and TILLING. Furthermore, deletions can be detected using oligomer chips suggesting a general technique to pinpoint deletions when genome-wide oligomer chips are broadly available. M4 mutant seeds are available for users for screening of altered response to multiple stresses. So far, more than 15,000 mutant lines have been distributed. To facilitate broad usage of the mutants, a mutant database has been constructed in the International Rice Information System (IRIS; http: //www.iris.irri.org) to document the phenotypes and gene function discovered by users.

Keywords

functional genomics mutagenesis Oryza sativa phenotype database 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Jian-Li Wu
    • 1
    • 2
  • Chanjian Wu
    • 1
  • Cailin Lei
    • 1
  • Marietta Baraoidan
    • 1
  • Alicia Bordeos
    • 1
  • Ma. Reina Suzette Madamba
    • 1
  • Marilou Ramos-Pamplona
    • 1
  • Ramil Mauleon
    • 1
  • Arlett Portugal
    • 3
  • Victor Jun Ulat
    • 3
  • Richard Bruskiewich
    • 3
  • Guoliang Wang
    • 4
  • Jan Leach
    • 5
  • Gurdev Khush
    • 1
  • Hei Leung
    • 1
    Email author
  1. 1.Entomology and Plant Pathology DivisionInternational Rice Research InstituteMetro ManilaPhilippines
  2. 2.National Center for Rice ImprovementChina National Rice Research InstituteHangzhouChina
  3. 3.Biometrics and Bioinformatics UnitInternational Rice Research InstituteMetro ManilaPhilippines
  4. 4.Department of Plant PathologyOhio State UniversityColumbusUSA
  5. 5.Department of Plant PathologyKansas State UniversityManhattanUSA

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