Phytochemistry Reviews

, Volume 16, Issue 6, pp 1253–1268 | Cite as

Bioactivities of Origanum vulgare L.: an update

  • Raffaele Pezzani
  • Sara Vitalini
  • Marcello Iriti


Origanum vulgare L. is a plant species belonging to the Lamiaceae and native to the Mediterranean region and western Eurasia. This aromatic herb is widely used throughout the world as spice and medicinal plant, representing a remedy in various traditional healing systems. In particular, oregano essential oil, rich in C10 monoterpenes, has been extensively used and, consequently, investigated. This narrative review aims to provide an updated overview on the biological activities of O. vulgare and its main bioactive phytochemicals, considering both preclinical (in vitro and in vivo) and in human studies focused on anticancer, anti-inflammatory, antioxidant and antimicrobial activities.


Biological activities Crude extracts Essential oil Oregano Preclinical studies 



This work was partially supported by Associazione Italiana per la Ricerca Oncologica di Base (AIROB, Padova, Italy).


  1. Akrayi HFS, Salih RMH, Hamad PA (2015) In vitro screening of antibacterial properties of Rhus coriaria and Origanum vulgare against some pathogenic bacteria. ARO. doi: 10.14500/aro.10085 Google Scholar
  2. Al-Kalaldeh JZ, Abu-Dahab R, Afifi FU (2010) Volatile oil composition and antiproliferative activity of Laurus nobilis, Origanum syriacum, Origanum vulgare, and Salvia triloba against human breast adenocarcinoma cells. Nutr Res 30:271–278CrossRefPubMedGoogle Scholar
  3. Assiri AMA, Elbanna K, Al-Thubiani A, Ramadan MF (2016) Cold-pressed oregano (Origanum vulgare) oil: a rich source of bioactive lipids with novel antoxidant and antimicrobial properties. Eur Food Res Technol 241:1013–1023CrossRefGoogle Scholar
  4. Begnini KR, Nedel F, Lund RG et al (2014) Composition and antiproliferative effect of essential oil of Origanum vulgare against tumor cell lines. J Med Food 17:1129–1133CrossRefPubMedGoogle Scholar
  5. Bennaoum Z, Benhassaini H, Falconieri D et al (2017) Chemical variability in essential oils from Ruta species among seasons, and its taxonomic and ecological significance. Nat Prod Res 31:2329–2334CrossRefPubMedGoogle Scholar
  6. Berrington D, Lall N (2012) Anticancer activity of certain herbs and spices on the cervical epithelial carcinoma (HeLa) cell line. Evid Based Complement Alternat Med. doi: 10.1155/2012/564927 PubMedPubMedCentralGoogle Scholar
  7. Bilen MA, Lin SH, Tang DG et al (2015) Maintenance therapy containing metformin and/or Zyflamend for advanced prostate cancer: a case series. Case Rep Oncol Med. doi: 10.1155/2015/471861 PubMedPubMedCentralGoogle Scholar
  8. Bukovska A, Cikos S, Juhas S et al (2007) Effects of a combination of thyme and oregano essential oils on TNBS-induced colitis in mice. Mediators Inflamm. doi: 10.1155/2007/23296 PubMedPubMedCentralGoogle Scholar
  9. Burt SA (2004) Essential oils: their antibacterial properties and potential applications in foods-a review. Int J Food Microbiol 94:223–253CrossRefPubMedGoogle Scholar
  10. Burt SA, van der Zeer R, Koets AP et al (2007) Carvacrol induces heat shock protein 60 and inhibits synthesis of flagellin in Escherichia coli O157:H7. Appl Environ Microbiol 73:4484–4490CrossRefPubMedPubMedCentralGoogle Scholar
  11. Carrasco A, Perez E, Cutillas AB et al (2016) Origanum vulgare and Thymbra capitata essential oils from Spain: determination of aromatic profile and bioactivities. Nat Prod Commun 11:113–120PubMedGoogle Scholar
  12. Chavan PS, Tupe SG (2014) Antifungal activity and mechanism of action of carvacrol and thymol against vineyard and wine spoilage yeasts. Food Control 46:115–120CrossRefGoogle Scholar
  13. Cirino ICS, Menezes-Silva SMP, Silva HTD et al (2014) The essential oil from Origanum vulgare L. and its individual constituents carvacrol and thymol enhance the effect of tetracycline against Staphylococcus aureus. Chemotherapy 60:290–293CrossRefGoogle Scholar
  14. Cleff MB, Madrid I, Meinerz AR et al (2017) Essential oils against Candida spp: in vitro antifungal activity of Origanum vulgare. Afric J Microbiol Res 7:2245–2250Google Scholar
  15. Coccimiglio J, Alipour M, Jiang ZH et al (2016) Antioxidant, antibacterial, and cytotoxic activities of the ethanolic Origanum vulgare extract and its major constituents. Oxid Med Cell Longev. doi: 10.1155/2016/1404505 PubMedPubMedCentralGoogle Scholar
  16. Dahiya P, Purkayastha S (2012) Phytochemical screening and antimicrobial activity of some medicinal plants against multi-drug resistant bacteria from clinical isolates. Indian J Pharm Sci 74:443–450CrossRefPubMedPubMedCentralGoogle Scholar
  17. De Falco E, Mancini E, Roscigno G et al (2013) Chemical composition and biological activity of essential oils of Origanum vulgare L. subsp. vulgare under different growth conditions. Molecules 18:14948–14960CrossRefPubMedGoogle Scholar
  18. De Falco E, Roscigno G, Landolfi S et al (2014) Growth, essential oil characterization, and antimicrobial activity of three wild biotypes of oregano under cultivation condition in Southern Italy. Ind Crops Prod 62:242–249CrossRefGoogle Scholar
  19. Economou G, Panagopoulos G, Karamanos A et al (2014) An assessment of the behavior of carvacrol–rich wild Lamiaceae species from the eastern Aegean under cultivation in two different environments. Ind Crops Prod 54:62–69CrossRefGoogle Scholar
  20. Fonseca AOS, Pereira DIB, Jacob RG et al (2015a) In vitro susceptibility of Brazilian Pythium insidiosum isolates to essential oils of some Lamiaceae family species. Mycophatologia 179:253–258CrossRefGoogle Scholar
  21. Fonseca AOS, Pereira DIB, Botton SA et al (2015b) In vitro susceptibility of Brazilian Pythium insidiosum isolates to essential oils of some Lamiaceae family species. Vet Microbiol 178:265–269CrossRefPubMedGoogle Scholar
  22. Fournomiti M, Kimbaris A, Mantzourani I et al (2015) Antimicrobial activity of essential oils of cultivated oregano (Origanum vulgare), sage (Salvia officinalis), and thyme (Thymus vulgaris) against clinical isolates of Escherichia coli, Klebsiella oxytoca, and Klebsiella pneumonia. Microb Ecol Health Dis. doi: 10.3402/mehd.v26.23289 PubMedPubMedCentralGoogle Scholar
  23. Fratini F, Mancini S, Turchi B et al (2017) A novel interpretation of the fractional inhibitory concentration index: the case of Origanum vulgare L. and Leptospermum scoparium J.R. et G. Forst essential oils against Staphylococcus aureus starins. Microbiol Res 195:11–17CrossRefPubMedGoogle Scholar
  24. Grbovic F, Stankovic MS, Curcic M et al (2013) In vitro cytotoxic activity of Origanum vulgare L. on HCT-116 and MDA-MB-231 cell lines. Plants (Basel) 2:371–378CrossRefGoogle Scholar
  25. Grondona E, Gatti G, Lopez AG et al (2014) Bio-efficacy of the essential oil of oregano (Origanum vulgare Lamiaceae. Ssp. hirtum). Plant Foods Hum Nutr 69:351–357CrossRefPubMedGoogle Scholar
  26. Gunawardena D, Shanmugam K, Low M et al (2014) Determination of anti-inflammatory activities of standardised preparations of plant- and mushroom-based foods. Eur J Nutr 53:335–343CrossRefPubMedGoogle Scholar
  27. Gupta RK, Patel AK, Shah N et al (2014) Oxidative stress and antioxidants in disease and cancer: a review. Asian Pac J Cancer Prev 15:4405–4409CrossRefPubMedGoogle Scholar
  28. Jnaid Y, Yacoub R, Al-Biski F (2016) Antioxidant and antimicrobial activities of Origanum vulgare essential oil. Int Food Res J 23:1706–1710Google Scholar
  29. Johnson CB, Kazantzis A, Skoula M et al (2004) Seasonal, populational and ontogenic variation in the volatile oil content and composition of individuals of Origanum vulgare subsp. hirtum, assessed by GC head-space analysis and by SPME sampling of individual oil glands. Phytochem Anal 15:286–292CrossRefPubMedGoogle Scholar
  30. Kačániová M, Vukovič N, Horská E et al (2014) Antibacterial activity against Clostridium genus and antiradical activity of the essential oils from different origin. J Environ Sci Health 49:505–512CrossRefGoogle Scholar
  31. Kelen M, Tepe B (2008) Chemical composition, antioxidant and antimicrobial properties of the essential oils of three Salvia species from Turkish flora. Bioresour Technol 99:4096–4104CrossRefPubMedGoogle Scholar
  32. Kogiannou DAA, Kalogeropoulos N, Kefalas P et al (2013) Herbal infusions; their phenolic profile, antioxidant and anti-inflammatory effects in HT29 and PC3 cells. Food Chem Toxicol 61:152–159CrossRefPubMedGoogle Scholar
  33. Kolberg M, Paur I, Balstad TR et al (2013) Plant extracts of spices and coffee synergistically dampen nuclear factor-kappaB in U937 cells. Nutr Res 33:817–830CrossRefPubMedGoogle Scholar
  34. Koldas S, Demirtas I, Ozen T et al (2015) Phytochemical screening, anticancer and antioxidant activities of Origanum vulgare L. ssp. viride (Boiss.) Hayek, a plant of traditional usage. J Sci Food Agric 95:786–798CrossRefPubMedGoogle Scholar
  35. Koukoulitsa C, Zika C, Hadjipavlou-Litina D et al (2006) Inhibitory effect of polar oregano extracts on aldose reductase and soybean lipoxygenase in vitro. Phytother Res 20:605–606CrossRefPubMedGoogle Scholar
  36. Kozlowska M, Laudy AE, Przybyl J et al (2015) Chemical composition and antibacterial activity of some medicinal plants from Lamiaceae family. Acta Pol Pharm 4:757–767Google Scholar
  37. Kubatka P, Kello M, Kajo K et al (2016) Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model. Eur J Nutr 56:1303–1316CrossRefPubMedGoogle Scholar
  38. Lesjack M, Simin N, Orcic D et al (2016) Binary and tertiary mixtures of Satureja hortensis and Origanum vulgare essential oils as potent antimicrobial agents against Helicobacter pylori. Phytother Res 30:476–484CrossRefGoogle Scholar
  39. Ličina BZ, Stefanović OD, Vasić SM et al (2013) Biological activity of the extracts from wild growing Origanum vulgare L. Food Control 33:498–504CrossRefGoogle Scholar
  40. Magi G, Marini E, Facinelli B (2015) Antimicrobial activity of essential oils and carvacrol and synergy of carvacrol and erythromycin-resistant Group A Streptococci. Front Microbiol. doi: 10.3389/fmicb.2015.00165 Google Scholar
  41. Maida I, Lo Nostro A, Pesavento G et al (2014) Exploring the anti-Burkholderia complex activity of essential oils: a preliminary analysis. Evid Based Complement Altern Med. doi: 10.1155/2014/573518 Google Scholar
  42. Marrelli M, Cristaldi B, Menichini F et al (2015) Inhibitory effects of wild dietary plants on lipid peroxidation and on the proliferation of human cancer cells. Food Chem Toxicol 86:16–24CrossRefPubMedGoogle Scholar
  43. Martins N, Barros L, Santos-Buelga C et al (2014) Decoction, infusion and hydroalcoholic extract of Origano vulgare L.: different performances regarding bioactivity and phenolic compounds. Food Chem 158:73–80CrossRefPubMedGoogle Scholar
  44. Mazzarrino G, Paparella A, Chaves-López C et al (2015) Salmorella enterica and Listeria monocytogenes inactivation dynamics after treatment with selected essential oils. Food Control 50:794–803CrossRefGoogle Scholar
  45. Mehreen A, Waheed M, Liaqat I et al (2016) Phytochemical. Antimicrobial, and toxicological evaluation of traditional herbs used to treat sore throat. Biomed Res Int, Article ID8503426Google Scholar
  46. Miller AB, Cates RG, Lawrence M et al (2015) The antibacterial and antifungal activity of theessential oils from Guatemalan medicinal plants. Pharm Biol 53:548–554CrossRefPubMedGoogle Scholar
  47. Misharina TA, Burlakova EB, Fatkullina LD et al (2013) Effect of oregano essential oil on the engraftment and development of Lewis carcinoma in F1 DBA C57 black hybrid mice. Prikl Biokhim Mikrobiol 49:423–428PubMedGoogle Scholar
  48. Moshayedi S, Shahraz F, Schaffner DW et al (2013) In vitro control of Enterococcus faecalis by Zataria multilfolira Boiss, Origanum vulgare L and Mentha pulegium essential oils. J Food Saf 33:327–332CrossRefGoogle Scholar
  49. Nagoor Meeran MF, Javed H, Taee HA et al (2017) Pharmacological properties and molecular mechanisms of thymol: prospects for its therapeutic potential and pharmaceutical development. Front Pharmacol 8:380CrossRefPubMedPubMedCentralGoogle Scholar
  50. NIH—National Institutes of Health (2017) Clinical TrialsGoogle Scholar
  51. Ocana-Fuentes A, Arranz-Gutierrez E, Senorans FJ et al (2010) Supercritical fluid extraction of oregano (Origanum vulgare) essentials oils: anti-inflammatory properties based on cytokine response on THP-1 macrophages. Food Chem Toxicol 48:1568–1575CrossRefPubMedGoogle Scholar
  52. Paur I, Balstad TR, Kolberg M et al (2010) Extract of oregano, coffee, thyme, clove, and walnuts inhibits NF-kappaB in monocytes and in transgenic reporter mice. Cancer Prev Res (Phila) 3:653–663CrossRefGoogle Scholar
  53. Pei RS, Zhou F, Ji BPETAL (2009) Evaluation of combined antibacterial effects of eugenol, cinnamaldehyde, thymol, and carvacrol against E. coli with an improved method. J Food Sci 74:379–383CrossRefGoogle Scholar
  54. Pesavento G, Maida I, Lo Nostro A et al (2016) Essential oil from Origanum vulgare completely inhibits the growth of multidrug-resistant cystic fibrosis pathogens. Nat Prod Commun 11:861–864PubMedGoogle Scholar
  55. Pezzani R, Rubin R, Monticelli H et al (2016) The usefulness of oregano’s phytocomplex crude extract: novel anti-proliferative effects in adrenocortical tumor cells. In: Paper presented at XXV Italo‐Latin American Congress of Ethnomedicine (SILAE), University of Modena and Reggio Emilia, Modena, 11–15 Sept 2016Google Scholar
  56. Pignatti S (1982) Flora d’Italia. Ed. Edagricole, Bologna, ItalyGoogle Scholar
  57. Ragi J, Pappert A, Rao B (2011) Oregano extract ointment for wound healing: a randomized, double-blind, petrolatum-controlled study evaluating efficacy. J Drugs Dermatol 10:1168–1172PubMedGoogle Scholar
  58. Rahman T, Hosen I, Islam M et al (2012) Oxidative stress and human health. Adv Biosci Biotechnol 3:997–1019CrossRefGoogle Scholar
  59. Rat MM, Gavaric NS, Kladar NV et al (2016) The phenolics of the Ornithogalum umbellatum L. (Hyacinthaceae): phytochemical and ecological characterization. Chem Biodivers 13:1551–1558CrossRefPubMedGoogle Scholar
  60. Rios JL, Recio MC, Escandell JM et al (2009) Inhibition of transcription factors by plant-derived compounds and their implications in inflammation and cancer. Curr Pharm Des 15:1212–1237CrossRefPubMedGoogle Scholar
  61. Şahin F, Gulluce M, Daierera D et al (2004) Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. vulgare in Eastern Anatolia region of Turkey. Food Control 15:549–557CrossRefGoogle Scholar
  62. Sánchez G, Aznar R (2015) Evaluation of natural compounds of plant origin for inactivation of enteric viruses. Food Environ Virol 7:183–187CrossRefGoogle Scholar
  63. Sankar R, Karthik A, Prabu A et al (2013) Origanum vulgare mediated biosynthesis of silver nanoparticles for its antibacterial and anticancer activity. Colloids Surf B Biointerfaces 108:80–84CrossRefPubMedGoogle Scholar
  64. Sarrou E, Tsivelika N, Chatzopoulou P et al (2017) Conventional breeding of Greek oregano (Origanum vulgare ssp. hirtum) and development of improved cultivars for yield potential and essential oil quality. Euphytica 213:104CrossRefGoogle Scholar
  65. Savini I, Arnone R, Catani MV et al (2009) Origanum vulgare induces apoptosis in human colon cancer caco2 cells. Nutr Cancer 61:381–389CrossRefPubMedGoogle Scholar
  66. Scandorieiro S, de Camargo LC, Lancheros CAC et al (2016) Synergistic and additive effect of oregano essential oil and biological silver nanoparticles against multidrug-resistant bacterial strains. Front Microbiol 7:1–14CrossRefGoogle Scholar
  67. Skendi A, Irakli M, Chatzopoulou P (2017) Analysis of phenolic compounds in Greek plants of Lamiaceae family by HPLC. J. Appl Res Med Aromat Plants. doi: 10.1016/j.jarmap.2017.02.001 Google Scholar
  68. Skotti E, Anastasaki E, Kanellou G et al (2014) Total phenolic content, antioxidant activity and toxicity of aqueous extracts from selected Greek medicinal and aromatic plants. Ind Crops Prod 53:46–54CrossRefGoogle Scholar
  69. Soares IH, Loreto ES, Rossato L et al (2015) In vitro activity of essential oil extracted from condiments against fluconazole-resistant and -sensitive Candida glabrata. J Mycol Med 25:213–217CrossRefPubMedGoogle Scholar
  70. Sommavilla V, Haidacher-Gasser D, Sgarbossa M et al (2012) Seasonal variation in phenolics in leaves of Celtis australis (Cannabaceae). Biochem Syst Ecol 41:110–114CrossRefGoogle Scholar
  71. Srihari T, Sengottuvelan M, Nalini N (2008a) Dose-dependent effect of oregano (Origanum vulgare L.) on lipid peroxidation and antioxidant status in 1,2-dimethylhydrazine-induced rat colon carcinogenesis. J Pharm Pharmacol 60:787–794CrossRefPubMedGoogle Scholar
  72. Srihari T, Balasubramaniyan V, Nalini N (2008b) Role of oregano on bacterial enzymes in 1,2-dimethylhydrazine-induced experimental colon carcinogenesis. Can J Physiol Pharmacol 86:667–674CrossRefPubMedGoogle Scholar
  73. Stelter K, Frahm J, Paulsen J et al (2013) Effects of oregano on performance and immunmodulating factors in weaned piglets. Arch Anim Nutr 67:461–476CrossRefPubMedGoogle Scholar
  74. Stojković D, Glamočlija J, Ćirić A et al (2013) Investigation on antibacterial synergism of Origanum vulgare and Thymus vulgaris essential oils. Arch Biol Sci 65:639–643CrossRefGoogle Scholar
  75. Teixeira B, Marques A, Ramos C et al (2013) Chemical composition and bioactivity of different oregano (Origanum vulgare) extracts and essential oil. J Sci Food Agric 93:2707–2714CrossRefPubMedGoogle Scholar
  76. Tusevski O, Kostovska A, Iloska A, Trajkovska L, Simic SG (2014) Phenolic production and antioxidant properties of some Macedonian medicinal plants. Centr Eur J Biol 9:888–900Google Scholar
  77. Valadez-Vega C et al (2013) The role of natural antioxidants in cancer disease. In: Morales González JA (ed) Oxidative stress and chronic degenerative diseases. InTech, Rijeka. doi: 10.5772/51503 Google Scholar
  78. Valente JS, Fonseca AOS, Denardi LB et al (2016) In vitro activity of antifungals in combination with essential oils against the oomycete Pythium insidiosum. J Appl Microbiol 121:998–1003CrossRefPubMedGoogle Scholar
  79. Vasko L, Vaskova J, Fejercakova A et al (2014) Comparison of some antioxidant properties of plant extracts from Origanum vulgare, Salvia officinalis, Eleutherococcus senticosus and Stevia rebaudiana. In Vitro Cell Dev Biol Anim 50:614–622CrossRefPubMedGoogle Scholar
  80. Vokou D, Kokkini S, Bessiere JM (1993) Geographic variation of Greek oregano (Origanum vulgare ssp. hirtum) essential oils. Biochem Syst Ecol 21:287–295CrossRefGoogle Scholar
  81. Vujicic M, Nikolic I, Kontogianni VG et al (2015) Methanolic extract of Origanum vulgare ameliorates type 1 diabetes through antioxidant, anti-inflammatory and anti-apoptotic activity. Br J Nutr 113:770–782CrossRefPubMedGoogle Scholar
  82. Waller SB, Madrid IM, Cleff MB et al (2016) Effects of essential oil of Rosmarinus officinalis Linn. and Origanum vulgare Linn. from different origins on Sporothrix brasiliensis and Sporothrix schenckii complex. Arq Bras Med Vet Zootec 68:991–999CrossRefGoogle Scholar
  83. WHO—World Health Organization (2017) Cancer—fact sheetGoogle Scholar
  84. Yaldiz G, Arici K, Yilmaz G (2017) Phytochemical analysis, antioxidant and antibacterial activities of four Lamiaceae species cultivated in barnyard manure. J Agric Sci 23:95–108Google Scholar
  85. Zhamanbayeva GT, Aralbayeva AN, Murzakhmetova MK et al (2016) Cooperative antiproliferative and differentiation-enhancing activity of medicinal plant extracts in acute myeloid leukemia cells. Biomed Pharmacother 82:80–89CrossRefPubMedGoogle Scholar
  86. Zhang YL, Pang LQ, Wu Y et al (2008) Significance of Bcl-xL in human colon carcinoma. World J Gastroenterol 14:3069–3073CrossRefPubMedPubMedCentralGoogle Scholar
  87. Zhao Y, Forst CV, Sayegh CE et al (2016) Molecular and genetic inflammation networks in major human diseases. Mol BioSyst 12:2318–2341CrossRefPubMedPubMedCentralGoogle Scholar

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Authors and Affiliations

  1. 1.O.U. Endocrinology, Department of Medicine (DIMED)University of PadovaPadovaItaly
  2. 2.AIROB, Associazione Italiana per la Ricerca Oncologica di BasePadovaItaly
  3. 3.Department of Agricultural and Environmental SciencesMilan State UniversityMilanoItaly

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