What can feruloyl esterases do for us?
- 916 Downloads
The role of feruloyl esterases in plant wall development, in gut health, and in the breakdown of plant biomass for the production of bioactive phytochemicals and biofuel is covered in this review. These enzymes have potential roles in stomatal cell function and the phenolic substitutions and cross-linkages between plant cell wall components. As more plant genomes are sequenced, the role of ferulic acid and feruloyl esterases in planta may be better understood. In human and ruminal digestion, these enzymes are important to de-esterify dietary fibre, releasing hydroxycinnamates and derivatives which have been shown to have positive health effects, such as antioxidant, anti-inflammatory and anti-microbial activities. They are also involved in colonic fermentation where their extracellular and intracellular activities in the microbiota improve the breakdown of polysaccharides and increase microbial production of short chain fatty acids. Their specificity can also be employed to synthesize bioactive compounds for cosmetic and health applications. The enzymatic disassembly of cereal straws is greatly enhanced when feruloyl esterase activity is present, although the substrate specificity of the esterase appears to have some bearing on its optimal application. The involvement of feruloyl esterases in the improved enzymatic and microbial saccharification of cereal-derived material demonstrates a high importance for these enzymes in animal feed preparation and bioalcohol production.
KeywordsPhenolic acids Human nutrition Biofuel Biomass breakdown Enzymatic synthesis
Type-A feruloyl esterase from Aspergillus niger
Type-B feruloyl esterase from Aspergillus niger
Cross-linked enzyme aggregate
Glycoside hydrolase family 10
Glycoside hydrolase family 11
One thousand Dalton units
Type-C feruloyl esterase from Sporotrichum thermosporum
Type-C feruloyl esterase from Talaromyces stipitatus
The Biotechnology and Biological Sciences Research Council (BBSRC), UK, is kindly thanked for financial support.
- Akin DE, Chesson A (1989) Lignification as the major factor limiting forage feeding value especially in warm conditions. Proc Int Grassl Congr 16:1753–1760Google Scholar
- Constabile A, Klinder A et al (2008) Whole-grain wheat breakfast cereal has a prebiotic effect on the human gut microbiota: a double-blind, placebo-controlled, crossover study. Br J Nutr 99:110–120Google Scholar
- Faulds CB (2003) Feruloyl esterases: molecular tools to unravel cell structure. Recent Res Devel Appl Microbiol Biotechnol 1:245–275Google Scholar
- Fazary AE, Ju YH (2008) The large-scale use of feruloyl esterases in industry. Biotechnol Mol Biol Rev 3:95–110Google Scholar
- Humberstone FJ, Briggs DE (2000) Extraction and assay of ferulic acid esterase from malted barley. J Inst Brew 106:21–29Google Scholar
- Kosuga M, Kosuga T et al (1998) Topical and cosmetic preparations containing capsaicins, sinapines, or curcumines for secretion stimulation. Eur Pat Appl A2 9 pp CODEN: JKXXAF JP 10120558 A2 19989512Google Scholar
- Moussou P, Danaoux L et al (2004) Use of sinapic acid and/or its derivatives for skin protection. Eur Pat Appl 17 pp CODEN;EPXXDW EP 1437117 A1 20040714Google Scholar
- Wong DW (2006) Feruloyl esterase: a key enzyme in biomass degradation. Appl Biochem Biophys 133:87–112Google Scholar