Genetic engineering of carotenoid formation in tomato
- 186 Downloads
The health promoting and aesthetic qualities of carotenoids have triggered considerable interest in enhancing their levels in crop plants, particularly in fruits and vegetables. One of the main crops of choice is the tomato, since it is readily available in both fresh and processed produce. There are several reports of the use of genetic engineering to increase levels of lycopene and \(\upbeta\)-carotene in ripe fruit. This review will highlight the strategies used to achieve these goals by comparing the use of different genes from plants and microorganisms as well as the choice of promoters.
KeywordsCarotenoids Isoprenoids Genetic modification Biosynthesis Metabolic regulation
Unable to display preview. Download preview PDF.
Financial support from the EU-FPIV (BI04 CT 97-2077), EU-FPV (QLK3-CT2000-0809) and UK Biotechnology and Biological Sciences Research Council is gratefully acknowledged. Wolfgang Schuch, Albert Boronat and Luisa-Maria Lois are thanked for their input, as is Syngenta.
- Bramley PM (2003a) The genetic enhancement of phytochemicals: the case of carotenoids. In: Johnson I, Williamson G (eds) Phytochemical functional foods, chapter 13. Woodhead Publishing Ltd, Cambridge, pp 253–279Google Scholar
- Bramley PM (2003b) Genetic engineering for carotenoids. In: Singh RP, Jaiwal PW (eds) Plant genetic engineering, vol 1. Sci Tech Publishing, Houston, pp 229–259Google Scholar
- Davuluri GR, van Tuinen A, Fraser PD, Manfredonia A, Newman R, Burgess D, Brummell DA, King SR, Palys J, Uhlig J, Bramley PM, Pennings HMJ, Bowler C (2005) Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes. Nat Biotech 23:890–895CrossRefGoogle Scholar
- Drake RG, Bird CR, Schuch W (1996) Enhancement of tomato phytoene synthase gene expression with a modified DNA. US Patent WO97/46690Google Scholar
- Giliberto L, Perrotta G, Pallara P, Weller JL, Fraser PD, Bramley PM, Fiore A, Tavazza M, Giuliano G (2005) Manipulation the blue light photoreceptor cryptochrome 2 in tomato affects vegetative development, flowering time and fruit nutritional quality. Plant Physiol 137:199–208PubMedCrossRefGoogle Scholar
- Mathews H, Clendennen SKJ, Caldwell CG, Liu XL, Connors K, Matheis N, Schuster DK, Menasco DJ, Wagoner W, Lightner J, Wagner DR (2003) Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis, modification and transport. Plant Cell 15:1689–1703PubMedCrossRefGoogle Scholar
- Reynard GB (1956) Origin of Webb Special (Black Queen) in tomato. Rep Tomato Genet Coop 6:22Google Scholar
- Soressi GP (1975) New spontaneous or chemically-induced fruit ripening tomato mutants. Rep Tomato Gent Coop 25:21–22Google Scholar
- Truesdale MR (1994) Carotenoid biosynthesis in the tomato. PhD thesis, University of LondonGoogle Scholar