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Photosynthetica

, Volume 56, Issue 4, pp 1447–1452 | Cite as

A method of a bicolor fast-Fourier pulse-amplitude modulation chlorophyll fluorometry

  • V. LysenkoEmail author
  • D. Lazár
  • T. Varduny
Brief Communication
  • 52 Downloads

Abstract

A simple method of a bicolor (multicolor), fast-Fourier, PAM chlorophyll fluorometry has been developed to obtain fluorescence induction curves. Quantum yields of PSII photochemistry were determined with blue and red simultaneously applied pulsed measuring lights for three subsequent 20-min periods of dark-, light-adaptation under actinic light and dark recovery. Measuring lights were cross-combined with blue and red actinic lights and saturation pulses. Coefficients of chromatic divergence were calculated as a ratio of the quantum yields obtained by red measuring light to that obtained by blue measuring light. Adaptation of Ficus benjamina and Hordeum vulgare leaves under blue (but not red) actinic light resulted in the sufficient increase of chromatic divergence. In addition, fraction of active, non(photo)inhibited, PSII centers was shown to be dependent on the color of measuring light. Thus, color of the light sources should be considered when reporting results of parameters evaluated from fluorescence induction curves.

Additional key words

chromatic adaptation greenhouse lighting dual-wavelength PAM multi-color PAM 

Abbreviations

AB and AR

blue and red actinic lights

AL

actinic light

Ft

operating or effective Chl fluorescence level in the light-adapted state

F0

minimal Chl fluorescence level in dark-adapted state

FFT

fast Fourier transform

Fm, Fm'

maximal Chl fluorescence level in dark-adapted and light-adapted state

Fm1'' and Fm2''

maximal Chl fluorescence levels recovered at the beginning and at the end of the second dark period

Fv

variable Chl fluorescence

LED

light-emitting diode

MB and MR

blue and red measuring flashes

ML

measuring light

SP

saturating pulse

PAM

pulse amplitude modulation (fluorometry)

SB and SR

blue and red saturating pulses

ξ

chromatic divergence of quantum yields of PSII photochemistry

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.Botanical Garden, Academy of Biology and BiotechnologySouthern Federal UniversityRostov-on-DonRussia
  2. 2.Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of SciencePalacký UniversityOlomoucCzech Republic

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