Photosynthetica

, Volume 56, Issue 1, pp 200–209 | Cite as

PsbY is required for prevention of photodamage to photosystem II in a PsbM-lacking mutant of Synechocystis sp. PCC 6803

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Abstract

The PsbM (3.9 kDa) and PsbY (4.2 kDa) proteins are membrane-spanning, single-helix, subunits associated with the chlorophyll-binding CP47 pre-complex of photosystem II (PSII). Removal of PsbM resulted in accumulation of PSII pre-assembly complexes and impaired electron transfer between the primary (QA) and secondary (QB) plastoquinone electron acceptors of PSII indicating that the QB-binding site and bicarbonate binding to the non-heme iron were altered in this strain. Removal of PsbY alone had only a minor impact on PSII activity but deleting PsbY in the ΔPsbM background led to additional modification of the acceptor side resulting in ΔPsbM:ΔPsbY cells being susceptible to photodamage and this required protein synthesis for recovery. Addition of bicarbonate was able to compensate for the light-induced damage in ΔPsbM:ΔPsbY cells potentially re-occupying the modified bicarbonate-binding site in the ΔPsbM:ΔPsbY strain and complementation of ΔPsbM:ΔPsbY cells with the psbY gene restored the ΔPsbM phenotype.

Additional key words

assembly bicarbonate chlorophyll fluorescence cytochrome b559 photoinhibition repair 

Abbreviations

Chl

chlorophyll

CP43

43 kDa chlorophyll-binding core antenna protein

CP47

47 kDa chlorophyll-binding core antenna protein

DCBQ

2,6-dichloro-1,4-benzoquinone

DCMU

3,4-dichloro-1,1-dimethyl urea

DMBQ

2,5-dimethyl-1,4- benzoquinone

F

fluorescence level

Fm

maximum fluorescence level

Fo

initial fluorescence level

HEPES

4-(2-hydroxyethyl)- 1-piperazineethanesulfonic acid

LMW

lte]ow molecular weight

PCC

Pasteur Culture Collection

QA

primary plastoquinone electron acceptor of PSII

QB

secondary plastoquinone electron acceptor of PS II

RC47

a PSII pre-assembly complex composed of the reaction center assembly module and the CP47 pre-complex assembly module

S2

the oxidation state of the oxygen-evolving complex following a single-turnover flash applied to dark-adapted cells

TES

2-[tris(hydroxymethyl)methyl]amino-1-ethanesulfonic acid

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.Department of BiochemistryUniversity of OtagoDunedinNew Zealand

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