Regulating photoprotection improves photosynthetic growth and biomass production in QC-site mutant cells of the cyanobacterium Synechocystis sp. PCC 6803
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We characterized the photosynthetic growth of wild-type (WT) and QC-site mutant cells of the cyanobacterium Synechocystis sp. PCC 6803 grown in a photobioreactor under medium-intensity [~70 μmol(photon) m–2 s–1] and high-intensity [~200 μmol(photon) m–2 s–1] light conditions. Photosynthetic growth rate (the exponential phase) increased about 1.1–1.2 fold for the A16FJ, S28Aβ, and V32Fβ mutant compared with WT cells under medium-intensity light and about 1.2–1.3 fold under high-intensity light. Biomass production increased about 17–20% for A16FJ and S28Aβ mutant cells as compared with WT cells under medium-intensity light and about 14–17% for A16FJ and V32Fβ mutant cells under high-intensity light. The greater photosynthetic growth rate and biomass production of these QC-site mutant cells could be attributed to the increased photosynthesis efficiency and decreased dissipation of wasteful energy from phycobilisomes in mutants vs. WT cells. Our results support that manipulation of photoprotection may improve photosynthesis and biomass production of photosynthetic organisms.
Additional key wordscytochrome b559 photosynthesis photosystem II photoprotection QC
the maximal fluorescence yield
the maximal fluorescence yield in the dark
the dark fluorescence yield
high- potential form
intermedium potential form
nonphotochemical fluorescence quenching
orange carotenoid proteins
wild-type control Synechocystis strain constructed in the same manner as site-directed mutants but with no mutation
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