, Volume 56, Issue 2, pp 567–577 | Cite as

Response of photosystem II performance and antioxidant enzyme activities in stay-green wheat to cytokinin

  • D. Q. Yang
  • Y. L. Luo
  • W. H. Dong
  • Y. P. Yin
  • Y. LiEmail author
  • Z. L. WangEmail author
Original paper


WN6 (a stay-green wheat cultivar) and JM20 (control) were used to evaluate the effects of exogenous cytokinin on photosynthetic capacity and antioxidant enzymes activities in flag leaves. Results showed that WN6 reached the higher grain mass, which was mainly due to the higher photosynthetic rate resulting from the higher maximal quantum yield of PSII photochemistry (ΦPSII) and probability that a trapped exaction transfers an electron into the electron transport chain beyond QA (Ψo), and lower relative variable fluorescence intensity at the J-step (Vj). Exogenous 6-benzylaminopurine (6-BA) enhanced antioxidant enzymes activities and decreased malondialdehyde (MDA) content. Enhanced Ψo and electron transport rate (ETR), and decreased Vj contributed to improved photosynthetic rate in the 6-BA treatment. In addition, exogenous 6-BA significantly increased endogenous zeatin (Zt) content, which was significantly and positively correlated with the antioxidant enzyme activity and ΦPSII, implying that higher Zt content was responsible for the improved antioxidant status and photosynthetic performance.

Additional key words

chlorophyll fluorescence gas exchange growth hormone JIP test performance index peroxidase 

Abbreviations (biaoti)


ascorbate peroxidase








days after anthesis


electron transport rate


the fluorescence at 30 ms


the fluorescence at 2 ms


the fluorescence at 300 μs


the fluorescence fluorescence at 20 μs


maximal fluorescence yield of the dark-adapted state


maximal fluorescence yield of the light-adapted state


steady-state fluorescence yield


fluorescence intensity at t time


variable fluorescence


maximal quantum yield of PSII photochemistry


fresh mass


stomatal conductance






performance index based on absorption of light energy


net photosynthetic rate




reactive oxygen species


stay-green cultivar


superoxide dismutase


relative variable fluorescence intensity at the J-step


relative variable fluorescence for the normalization between Fo and Fk




effective quantum yield of PSII photochemistry


probability that a trapped exaction transfers an electron into the electron transport chain beyond QA.


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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Crop BiologyShandong Agricultural UniversityTai’an, ShandongChina

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