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Photosynthetica

, Volume 54, Issue 3, pp 414–421 | Cite as

Effects of drought stress on fluorescence characteristics of photosystem II in leaves of Plectranthus scutellarioides

  • L.-L. Meng
  • J.-F. Song
  • J. Wen
  • J. Zhang
  • J.-H. Wei
Original papers

Abstract

Drought stress has multiple effects on the photosynthetic apparatus. Herein, we aimed to study the effect of drought stress on fluorescence characteristics of PSII in leaves of Plectranthus scutellarioides and explore potentially underlying mechanisms. Plants of P. scutellarioides were grown in a greenhouse and subjected to drought (DS, drought-stressed) or daily irrigation (control group). Leaf chlorophyll (Chl) index and induction kinetics curves of Chl a fluorescence and the JIP-test were used to evaluate effects of drought lasting for 20 d. Our results showed that both the leaf and soil relative water content decreased with increasing treatment duration. The leaf Chl index was reduced to half in the DS plants compared with the control group after 20 d. The minimal fluorescence in the DS plants was higher than that in the control plants after 10 d of the treatment. Maximum photochemical efficiency and lateral reactivity decreased with increasing treatment duration in the DS plants. With the continuing treatment, values of absorption flux per reaction center (RC), trapped energy flux per RC, dissipated energy flux per RC, and electron transport flux per RC increased in the earlier stage in the DS plants, while obviously decreased at the later stage of the treatment. In conclusion, drought stress inhibited the electron transport and reduced PSII photochemical activity in leaves of P. scutellarioides.

Additional key words

Coleus electron transport fluorescence transient performance index photoinhibition 

Abbreviations

ABS/RC

quantum yield of electron transport

DAT

day of treatment

DI0/RC

trapped energy flux per reaction center

DM

dry mass

DS

drought-stressed

ET0/RC

absorption flux per reaction center

F0

minimal recorded fluorescence intensity when all PSII reaction centers are open

FM

fresh mass

Ft

fluorescence intensity at t time

LRWC

leaf relative water content

M0

approximated initial slope of the fluorescence transient

OEC

oxygen evolving complex

PIabs

dissipated energy flux per reaction center

PQ

plastoquinone

QA

primary quinone acceptor

QB

secondary quinone acceptor

RC/ABS

electron transport flux per reaction center

RCs

reaction centers

Sm

normalized total complementary area above the OJIP transient

SWC

soil water content

TM

turgid mass

TR0/RC

quantum yield of dissipation

VJ

relative variable fluorescence intensity at the J-step

WOJ

relative variable fluorescence for the normalization between F0 and FJ

WOK

relative variable fluorescence for the normalization between F0 and F300µs

ψ0

lateral reactivity of PSII.

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Copyright information

© The Institute of Experimental Botany 2016

Authors and Affiliations

  • L.-L. Meng
    • 1
  • J.-F. Song
    • 2
  • J. Wen
    • 3
  • J. Zhang
    • 4
  • J.-H. Wei
    • 5
  1. 1.Institute of Agricultural Facilities and EquipmentJiangsu Academy of Agricultural SciencesNanjing, Jiangsu ProvinceChina
  2. 2.Institute of Farm Product ProcessingJiangsu Academy of Agricultural SciencesNanjing, Jiangsu ProvinceChina
  3. 3.Institute of HorticultureJiangsu Academy of Agricultural SciencesNanjing, Jiangsu ProvinceChina
  4. 4.Institute of Vegetable CropsJiangsu Academy of Agricultural SciencesNanjing, Jiangsu ProvinceChina
  5. 5.Science and Technology IndustryJiangsu Academy of Agricultural SciencesNanjing, Jiangsu ProvinceChina

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