, Volume 54, Issue 3, pp 422–429 | Cite as

Chlorophyll a fluorescence analysis of high-yield rice (Oryza sativa L.) LYPJ during leaf senescence

  • Y. W. Wang
  • C. Xu
  • C. F. Lv
  • M. Wu
  • X. J. Cai
  • Z. T. Liu
  • X. M. Song
  • G. X. Chen
  • C. G. Lv
Original papers


Photosystem II (PSII) photochemistry was examined by chlorophyll (Chl) a fluorescence analysis in high-yield rice LYPJ flag leaves during senescence. Parameters deduced from the JIP-test showed that inhibition of the donor side of PSII was greater than that of the acceptor side in hybrid rice LYPJ. The natural senescence process was accompanied by the increased inactivation of oxygen-evolving complex (OEC) and a lower total number of active reaction centers per absorption. It indicated that the inhibition of electron transport caused by natural senescence might be caused partly by uncoupling of the OEC and/or inactivation of PSII reaction centers. Chl fluorescence parameters analyzed in this study suggested that energy dissipation was enhanced in order to protect senescent leaves from photodamage. Nevertheless, considerably reduced PSI electron transport activity was observed at the later senescence. Thus, natural senescence inhibited OEC-PSII electron transport, but also significantly limited the PSII-PSI electron flow.

Additional key words

JIP-test natural senescence PSII efficiency thylakoid membrane 





cross section


the total driving force for photosynthesis of the observed system


fluorescence intensity at 50 µs


fluorescence intensity at the J-step (at 2 ms)


fluorescence intensity at the I-step (at 30 ms)


maximal fluorescence intensity


maximum photochemical efficiency of PSII


oxygen-evolving complex


primary electron donor in PSII


the performance index on cross section basis at different times


the potential for energy conservation from photons absorbed by PSII to the reduction flux (RE) of PSI end acceptors




PSII reaction center


time to reach FM


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Copyright information

© The Institute of Experimental Botany 2016

Authors and Affiliations

  • Y. W. Wang
    • 1
  • C. Xu
    • 1
  • C. F. Lv
    • 1
  • M. Wu
    • 1
    • 2
  • X. J. Cai
    • 1
  • Z. T. Liu
    • 1
  • X. M. Song
    • 1
  • G. X. Chen
    • 1
  • C. G. Lv
    • 3
  1. 1.Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences CollegeNanjing Normal UniversityNanjingChina
  2. 2.Zijin CollegeNanjing University of Science and TechnologyNanjingChina
  3. 3.Institute of Food and CropsJiangsu Academy of Agricultural SciencesNanjingChina

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