Growth irradiance affects ureide accumulation and tolerance to photoinhibition in Eutrema salsugineum (Thellungiella salsuginea)
- 229 Downloads
Plants are able to acclimate to their growth light environments by utilizing a number of short- and long-term mechanisms. One strategy is to prevent accumulation of excess reactive oxygen species that can lead to photoinhibition of photosynthesis. Ureides, generated from purine degradation, have been proposed as antioxidants and involved in certain abiotic stress responses. Eutrema salsugineum (Thellungiella salsuginea) is an extremophilic plant known to exhibit a high degree of tolerance to a variety of abiotic stresses that invariably generate reactive oxygen species. In the present study we have investigated the possible role of the ureide metabolic pathway during acclimation to growth irradiance and its conference of tolerance to photoinhibition in Eutrema. Ureide accumulation was greater under high light growth which also conferred tolerance to photoinhibition at low temperature as measured by the maximal quantum yield of PSII photochemistry. This may represent an adaptive plastic response contributing to the extreme tolerance exhibited by this plant. Our results would provide evidence that ureide accumulation may be involved in abiotic stress as another defence mechanism in response to oxidative stress.
Additional key wordsacclimation allantoin antioxidant reactive oxygen species
maximal quantum yield of PSII photochemistry
reactive oxygen species
semiquantitative reverse transcriptase-polymerase chain reaction
Unable to display preview. Download preview PDF.
- Anderson J.M., Osmond C.B.: Shade-sun responses: compromises between acclimation and photoinhibition. — In: Kyle D.J., Osmond C.B., Arntzen C.J. (ed.): Topics in Photosynthesis, Vol. 9. Photoinhibition. Pp. 1–38. Elsevier, Amsterdam 1987.Google Scholar
- Asada K., Takahashi M.: Production and scavenging of active oxygen in photosynthesis. — In: Kyle D.J., Osmond C.B., Arntzen C.J. (ed.): Topics in Photosynthesis, Vol. 9, Photoinhibition. Pp. 227–287. Elsevier, Amsterdam 1987.Google Scholar
- Castro A.H.F., Young M.C.M., de Alvarenga A.A., Alves J.D.: Influence of photoperiod on the accumulation of allantoin in comfrey plants. — R. Bras. Fisiol. Veg. 13: 49–54, 2001.Google Scholar
- Kansal R., Kuhar K., Verma I. et al.: Improved and convenient method of RNA isolation from polyphenols and polysaccharide rich plant tissues. — Indian J. Exp. Biol. 47: 842–845, 2008.Google Scholar
- Karuppanapandian T., Moon J.C., Kim C. et al.: Reactive oxygen species in plants: their generation, signal transduction, and scavenging mechanisms. — Aust. J. Crop Sci. 5: 709–725, 2011.Google Scholar
- Sambrook J., Russell D.W.: Molecular Cloning: A Laboratory Manual. 3rd Edition. Pp. 2100. Cold Spring Harbor Laboratory Press, New York 2001.Google Scholar
- Serraj R., Sinclair T.R., Purcell L.C.: Symbiotic N2 fixation response to drought. — J. Exp. Bot. 50: 143–155, 1999.Google Scholar