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Photosynthetica

, Volume 52, Issue 4, pp 564–573 | Cite as

Comparative analysis of drought stress effects on photosynthesis of Eurasian and North African genotypes of wild barley

  • C. Jedmowski
  • S. Bayramov
  • W. Brüggemann
Original Papers

Abstract

The impact of drought stress (DS) on eight Eurasian and North African genotypes of wild barley (Hordeum spontaneum) was evaluated by analysis of chlorophyll (Chl) a fluorescence fast induction curves using the JIP-test. Three-week-old, pot-grown plants were exposed to a DS treatment by withholding water for nine days. The genotype-specific impairment of the functionality of the photosynthetic electron transport chain was quantified using the relative decline of the performance indices (PIabs and PItot), two key parameters of the JIP-test. The genotypes showing the highest (HOR10164) and lowest (HOR10710) relative PIs under DS were subjected to additional experiments, including measurements of leaf gas exchange, water status, pigment content, key enzyme activity, and protein abundance. The genotypes showed a specific profile of DS-mediated inhibition of photosynthesis, associated with higher relative leaf water contents in HOR10164 at the end of the treatment. Whereas decreased photosynthetic rate in HOR10164 was mainly caused by stomatal closure, nonstomatal limitations (decreased Rubisco content and activity) were detected in HOR10710. Additional genotype specific features were the upregulation of the NADP-malate dehydrogenase in HOR10164 and a decreased fraction of QA-reducing reaction centers in HOR10710.

Additional key words

Calvin cycle chlorophyll fluorescence JIP-test performance index photosynthetic CO2 assimilation 

Abbrevations

Ci

intercellular CO2 concentration

Car

carotenoids

Chl

chlorophyll

cpHSP70

plastidic heat shock protein 70

Dhn1

dehydrin 1

DM

dry mass

DS

drought stress

FM

fresh mass

gs

stomatal conductance

LA

leaf area

LSU

large subunit

NADP-MDH

NADP-malate dehydrogenase

PN

net photosynthesis rate

PI

performance index

PsbO

psbO — manganese stabilising protein of the oxygen evolving complex of PSII

RA

Rubisco activase

RC

reaction center

RC/ABS

fraction of active (QA-reducing) PSII reaction centers

ROS

reactive oxygen species

RWC

relative water content (of leaves)

sFBPase

stromal fructose-1,6-bisphosphatase

SWC

soil water content

TM

turgid mass

VI

relative variable fluorescence at the I-step (30 ms)

VJ

relative variable fluorescence at the J-step (2 ms)

δRo

efficiency of electron transport from the intersystem electron carriers to the PSI acceptor side

ϕPo

maximum photochemical efficiency of PSII

Ψsoil

soil water potential

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Copyright information

© The Institute of Experimental Botany 2014

Authors and Affiliations

  1. 1.Department of Ecology, Evolution and DiversityUniversity of FrankfurtFrankfurtGermany
  2. 2.Institute of BotanyAzerbaijan National Academy of SciencesBakuAzerbaijan
  3. 3.Biodiversity and Climate Research CenterFrankfurtGermany

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