, Volume 52, Issue 3, pp 475–478 | Cite as

Sodium bisulfite enhances photosynthesis in rice by inducing Rubisco activase gene expression

  • Y. Chen
  • J. -H. Jin
  • Q. -S. Jiang
  • C. -L. Yu
  • J. Chen
  • L. -G Xu
  • D. -A. Jiang
Brief Communication


To investigate how bisulfite promotes photosynthesis, a pot experiment was conducted with rice (Oryza sativa L.) plants to determine Rubisco activity and content, and Rubisco activase (RCA) gene expression after spraying NaHSO3 on rice leaves. The NaHSO3 treatment promoted significantly net photosynthetic rate (P N), carboxylation efficiency, maximum carboxylation rate, ribulose-1,5-bisphosphate regeneration rate, initial Rubisco activity, and RCA protein and mRNA concentrations. Therefore, the NaHSO3 enhancement of P N could be directly attributed to induction of RCA gene expression both at the transcription and translation levels. Thus, the increased RCA regulated the initial Rubisco activity in vivo.

Additional key words

carboxylation efficiency grain yield maximum carboxylation rate photorespiration RuBP regeneration rate 



apparent quantum yield


carboxylation efficiency


enzyme-linked immunosorbent assay


intercellular CO2 concentration


stomatal conductance


RuBP regeneration rate


net photosynthetic rate


Rubisco activase


RCA large isoform


RCA small isoform


dark respiration rate


Rubisco large subunit


photorespiration rate


Rubisco small subunit




maximum carboxylation rate


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Copyright information

© The Institute of Experimental Botany 2014

Authors and Affiliations

  • Y. Chen
    • 1
  • J. -H. Jin
    • 1
  • Q. -S. Jiang
    • 1
  • C. -L. Yu
    • 1
  • J. Chen
    • 2
  • L. -G Xu
    • 1
  • D. -A. Jiang
    • 1
  1. 1.State Key Laboratory of Plant Physiology and Biochemistry, College of Life SciencesZhejiang UniversityHangzhouChina
  2. 2.Canal Street AgencyYuhang District People’s GovernmentHangzhouChina

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