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Photosynthetica

, Volume 52, Issue 3, pp 475–478 | Cite as

Sodium bisulfite enhances photosynthesis in rice by inducing Rubisco activase gene expression

  • Y. Chen
  • J. -H. Jin
  • Q. -S. Jiang
  • C. -L. Yu
  • J. Chen
  • L. -G Xu
  • D. -A. Jiang
Brief Communication

Abstract

To investigate how bisulfite promotes photosynthesis, a pot experiment was conducted with rice (Oryza sativa L.) plants to determine Rubisco activity and content, and Rubisco activase (RCA) gene expression after spraying NaHSO3 on rice leaves. The NaHSO3 treatment promoted significantly net photosynthetic rate (P N), carboxylation efficiency, maximum carboxylation rate, ribulose-1,5-bisphosphate regeneration rate, initial Rubisco activity, and RCA protein and mRNA concentrations. Therefore, the NaHSO3 enhancement of P N could be directly attributed to induction of RCA gene expression both at the transcription and translation levels. Thus, the increased RCA regulated the initial Rubisco activity in vivo.

Additional key words

carboxylation efficiency grain yield maximum carboxylation rate photorespiration RuBP regeneration rate 

Abbreviations

AQY

apparent quantum yield

CE

carboxylation efficiency

ELISA

enzyme-linked immunosorbent assay

Ci

intercellular CO2 concentration

gs

stomatal conductance

Jmax

RuBP regeneration rate

PN

net photosynthetic rate

RCA

Rubisco activase

RCAL

RCA large isoform

RCAs

RCA small isoform

RD

dark respiration rate

RLS

Rubisco large subunit

RP

photorespiration rate

RSS

Rubisco small subunit

RuBP

ribulose-1,5-bisphosphate

Vcmax

maximum carboxylation rate

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Copyright information

© The Institute of Experimental Botany 2014

Authors and Affiliations

  • Y. Chen
    • 1
  • J. -H. Jin
    • 1
  • Q. -S. Jiang
    • 1
  • C. -L. Yu
    • 1
  • J. Chen
    • 2
  • L. -G Xu
    • 1
  • D. -A. Jiang
    • 1
  1. 1.State Key Laboratory of Plant Physiology and Biochemistry, College of Life SciencesZhejiang UniversityHangzhouChina
  2. 2.Canal Street AgencyYuhang District People’s GovernmentHangzhouChina

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