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Photosynthetica

, Volume 52, Issue 2, pp 288–300 | Cite as

The potential of leaf chlorophyll content to screen bread-wheat genotypes in saline condition

  • A. Kiani-Pouya
  • F. Rasouli
Article

Abstract

Physiological traits, which are positively associated with yield under salt-stress conditions, can be useful selection criteria in screening for salt tolerance. We examined whether chlorophyll (Chl) content can be used as screening criterion in wheat. Our study involved 5 wheat genotypes under both saline and nonsaline field conditions as well as in a sand-culture experiment. Salt stress reduced significantly biomass, grain yield, total Chl and both Chl a and b in all genotypes. In the sand-culture experiment, Chl accumulation was higher in PF70354/BOW, Ghods, and H499.71A/JUP genotypes at nonsaline control, moderate, and high salt concentrations, respectively. In the field experiment, genotype H499.71A/JUP belonged to those with the highest Chl density. The SPAD (Soil Plant Analysis Development) meter readings were linearly related to Chl content both in the sand-culture and in the field experiment. However, salt stress affected the calibration of SPAD meter. Therefore, separate Chl-SPAD equations were suggested for saline and nonsaline conditions. The correlation coefficients between the grain yield and SPAD were positive and significant both in the sand culture and in the field experiment. These findings suggested that SPAD readings could be used as a tool for rapid assessment of relative Chl content in wheat genotypes. It could be used for the indirect selection of high-yielding genotypes of wheat under saline condition in sand-culture and field experiments.

Additional key words

biomass NaCl SPAD Triticum aestivum L. 

Abbreviations

AN

anthesis

BM

biomass

EC

electrical conductivity

ET

early tillering

GF

grain filling

GY

grain yield

Chl

chlorophyll

HS

high salt stress

LT

late tillering

MS

moderate salt stress

NSA

nonsaline conditions

SPAD

Soil Plant Analysis Development

TChl

total chlorophyll

S0

0 mM NaCl+CaCl2

S100

100 mM NaCl+CaCl2

S200

200 mM NaCl+CaCl2

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Copyright information

© The Institute of Experimental Botany 2014

Authors and Affiliations

  1. 1.Department of Salinity ResearchResearch Center for Agriculture and Natural ResourcesFarsIran

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