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Photosynthetica

, Volume 52, Issue 2, pp 223–232 | Cite as

Effects of light intensity and temperature on the photosynthetic irradiance response curves and chlorophyll fluorescence in three picocyanobacterial strains of Synechococcus

  • S. Jodłowska
  • S. Śliwińska
Article

Abstract

Chrococcoid cyanobacteria of the genus Synechococcus are the important component of marine and freshwater ecosystems. Picocyanobacteria comprise even 80% of total cyanobacterial biomass and contribute to 50% of total primary cyanobacterial bloom production. Chlorophyll (Chl) fluorescence and photosynthetic light response (P-I) curves are commonly used to characterize photoacclimation of Synechococcus strains. Three brackish, picocyanobacterial strains of Synechococcus (BA-132, BA-124, BA-120) were studied. They were grown under 4 irradiances [10, 55, 100, and 145 μmol(photon) m−2 s−1] and at 3 temperatures (15, 22.5, and 30°C). Photosynthetic rate was measured by Clark oxygen electrode, whereas the Chl fluorescence was measured using Pulse Amplitude Modulation fluorometer. Based on P-I, two mechanisms of photoacclimation were recognized in Synechococcus. The maximum value of maximum rate of photosynthesis (P max) expressed per biomass unit at 10 μmol(photon) m−2 s−1 indicated a change in the number of photosynthetic units (PSU). The constant values of initial slope of photosynthetic light response curve (α) and the maximum value of P max expressed per Chl unit at 145 μmol(photon) m−2 s−1 indicated another mechanism, i.e. a change in PSU size. These two mechanisms caused changes in photosynthetic rate and its parameters (compensation point, α, saturation irradiance, dark respiration, P max) upon the influence of different irradiance and temperature. High irradiance had a negative effect on fluorescence parameters, such as the maximum quantum yield and effective quantum yield of PSII photochemistry (φPSII), but it was higher in case of φPSII.

Additional key words

fluorescence irradiance photoacclimation photosynthesis picocyanobacteria temperature 

Abbreviations

ANOVA

analysis of variance

CCBA

Culture Collection of Baltic Algae

Chl

chlorophyll

IK

saturation irradiance

FV/FM

maximum quantum yield of PSII photochemistry

N

number of cells

OD

optical density

PAR

photosynthetically active radiation

PAM

Pulse Amplitude Modulation

CP

compensation point

P-I

photosynthetic light response

Pmax

maximum photosynthetic rate

PN

net photosynthetic rate

PSU

photosynthetic units

RD

dark respiration rate

TEM

transmission electron microscope

ΦPSII

effective quantum yield of PSII photochemistry

α

initial slope of photosynthetic light response curve

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Copyright information

© The Institute of Experimental Botany 2014

Authors and Affiliations

  1. 1.Laboratory of Marine Plant Ecophysiology, Institute of OceanographyUniversity of GdańskGdyniaPoland

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