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Photosynthetica

, Volume 52, Issue 2, pp 193–202 | Cite as

Different drought-stress responses in photosynthesis and reactive oxygen metabolism between autotetraploid and diploid rice

  • P. -M. Yang
  • Q. -C. Huang
  • G. -Y. Qin
  • S. -P. Zhao
  • J. -G. Zhou
Article

Abstract

Photosynthetic light curve, chlorophyll (Chl) content, Chl fluorescence parameters, malondialdehyde (MDA) content, phosphoenolpyruvate carboxylase (PEPC) activity and reactive oxygen metabolism were studied under drought stress in two autotetraploid rice lines and corresponding diploid rice lines. Net photosynthetic rate decreased dramatically, especially under severe drought stress and under high photosynthetic active radiation in diploid rice, while it declined less under the same conditions in autotetraploid lines. Compared with the corresponding diploid lines, the Chl content, maximum photochemical efficiency of photosystem (PS) II, and actual photochemical efficiency of PSII were reduced less in autotetraploid lines. PEPC activities were higher in autotetraploid rice lines. PEPC could alleviate inhibition of photosynthesis caused by drought stress. The chromosome-doubling enhanced rice photoinhibition tolerance under drought stress. The lower MDA content and superoxide anion production rate was found in the autotetraploid rice indicating low peroxidation level of cell membranes. At the same time, the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities were higher in autotetraploid rice lines. SOD, POD, and CAT could effectively diminish the reactive oxygen species and reduced the membrane lipid peroxidation.

Additional key words

chlorophyll fluorescence Oryza sativa photosynthetic light curve reactive oxygen species 

Abbreviations

CAT

catalase

Chl

chlorophyll

DM

dry mass

Fv/Fm

maximum photochemical efficiency of PSII

FM

fresh mass

MDA

malondialdehyde

O2·−

superoxide anion

PEPC

phosphoenolpyruvate carboxylase

PN

net photosynthetic rate

POD

peroxidase

PS

photosystem

ROS

reactive oxygen species

SOD

superoxide dismutase

ΦPSII

actual photochemical efficiency of PSII

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • P. -M. Yang
    • 1
    • 2
  • Q. -C. Huang
    • 1
  • G. -Y. Qin
    • 1
  • S. -P. Zhao
    • 1
  • J. -G. Zhou
    • 2
  1. 1.Henan Provincial Key Laboratory of Ion Beam Bio-engineeringZhengzhou UniversityZhengzhou, HenanChina
  2. 2.Henan Institute of Science and TechnologyXinxiang, HenanChina

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