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Photosynthetica

, Volume 49, Issue 4, pp 497–506 | Cite as

Photoprotective function of betacyanin in leaves of Amaranthus cruentus L. under water stress

  • T. NakashimaEmail author
  • T. Araki
  • O. Ueno
Original Papers

Abstract

The photoprotective function of leaf betacyanin in water-stressed Amaranthus cruentus plants was examined by comparing leaves of two strains which differ significantly in the amount of betacyanin. At 0, 1, and 2 days after the imposed water stress, leaves were subjected to high-light (HL) treatment to assess their photosynthetic capacity and photoinhibition susceptibility. The water stress equally reduced leaf relative water content (RWC), gas-exchange rate and chlorophyll (Chl) contents in both leaves, indicating that the severity of water stress was comparable between the strains. Consequently, the extent of photoinhibition after the HL treatment increased in both strains as water stress developed; however, it was significantly greater in acyanic leaves than in betacyanic leaves, suggesting lower photoinhibition susceptibility in the betacyanic strain. The betacyanic leaves also exhibited approximately 30% higher values for photochemical quenching coefficient (qP) during the period of water stress despite the nonphotochemical quenching coefficient (qN) did not differ significantly between the strains. These results may be partially explained by the increased amount of leaf betacyanin under water stress. Moreover, a decrease in Chl content in betacyanic leaves might have enhanced light screening effect of betacyanin by increasing relative abundance of betacyanin to Chl molecule. In addition, reduced Chl content increased light penetrability of leaves. As a result, the extent of photoinhibition at the deeper tissue was exacerbated and the Chl fluorescence emitted from these tissues was more readily detected, facilitating assessment of photoinhibition at deeper tissues where the effect of betacyanic light screening is considered to be most apparent. Our results demonstrated that leaf betacyanin contributes to total photoprotective capacity of A. cruentus leaves by lowering excitation pressure on photosystem II (PSII) via attenuation of potentially harmful excess incident light under water stress.

Additional key words

betacyanin grain amaranthus light screening maximum quantum yield of photosystem II photoinhibition water deficit 

Abbreviations

Chl

chlorophyll

DAT

day(s) after treatment

ΔT

difference in transmission spectra between the strains

F0 and Fm

minimum and maximum fluorescence yield of dark-adapted leaves, respectively

Fv

variable fluorescence in dark-adapted state

Fm′, F0′, and Fs

maximum, minimum, and steady-state fluorescence yield of light-adapted leaves, respectively

Fv

variable fluorescence in light-adapted state

Fv/Fm

maximum quantum yield of PSII

gs

stomatal conductance

HL

high light

PIFv/Fm

percent inhibition of Fv/Fm after HL treatment

PN

net photosynthetic rate

PPFD

photosynthetic photon flux density

PS

photosystem

QA

primary quinone acceptor of PSII

qN and qP

nonphotochemical and photochemical quenching coefficient, respectively

ROS

reactive oxygen species

RWC

leaf relative water content

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Notes

Acknowledgements

We thank Dr. M. Katsuta (National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Japan) for kindly providing seeds of A. cruentus, Dr. S. Agarie (Faculty of Agriculture, Kagawa University, Kagawa, Japan) for lecturing us the method of betacyanin quantification, and Dr. S. Yamashita (Faculty of Agriculture, Kyushu University, Fukuoka, Japan) for allowing us to use the spectroscope.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Graduate School of Bioresource and Bioenvironmental SciencesKyushu UniversityFukuokaJapan
  2. 2.Faculty of AgricultureEhime UniversityEhimeJapan
  3. 3.Faculty of AgricultureKyushu UniversityFukuokaJapan

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