Photosynthetica

, Volume 49, Issue 1, pp 140–144 | Cite as

Photosynthetic characterization at different senescence stages in an early senescence mutant of rice Oryza sativa L.

Brief Communication

Abstract

An early senescence (es) mutant of rice Oryza sativa L. with progressing death of most of leaves before heading stage was identified in the field in Hainan province. After tillering stage, the brown striations were found in the base of green leaves randomly, and then expanded to whole leaves. No fungi, bacteria, and viruses were detected in the brown striations suggesting that it was a genetic mutant. The ultrastructure of leaf cells at the site of brown striations showed breakdown of chloroplast thylakoid membrane structures and other organelles, and condensation of the cytoplasm at severe senescence stage. The photosynthetic activity and chlorophyll (Chl) contents decreased irreversibly along with leaf senescence process.

Additional key words

photosynthesis pigments senescence ultrastructure 

Abbreviations

Chl

chlorophyll

es

early senescence

F0

minimal fluorescence level in dark-adapted leaves

F0

minimal fluorescence level in light-adapted leaves

Fm

maximal fluorescence level in dark-adapted leaves

Fm

maximal fluorescence level in light-adapted leaves

Fv

variable fluorescence level in dark-adapted leaves

Fv

variable fluorescence level in light-adapted leaves

Fv/Fm

maximal efficiency of PSII photochemistry

Fv′/Fm

efficiency of excitation energy capture by open PSII reaction centers

MDA

malondialdehyde

NBT

nitroblue tetrazolium

NPQ

nonphotochemical quenching

POD

peroxidase

PPFD

photosynthetic photon flux density

PSII

photosystem II

qP

photochemical quenching coefficient

SOD

superoxide dismutase

β-Car

β-carotene

ΦPSII

actual PSII efficiency

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Notes

Acknowledgements

This work is supported by Chinese National Nonprofit Institute Research Grant of CATAS-RRI (YWFZX09-02(N)) and Supported by an earmarked fund for Modern Agro-industry Technology Research System (No.nycytx-34-GW1-2).

References

  1. Alscher, R.G., Erturk, N., Heath, L.S.: Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. — J. Exp. Bot. 53: 1331–1341, 2002.PubMedCrossRefGoogle Scholar
  2. Apel, K., Hirt, H.: Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. — Annu. Rev. Plant. Biol. 55: 373–399, 2004.PubMedCrossRefGoogle Scholar
  3. Aust, S.D., Morehouse, L.A., Thomas, C.E.: Role of metals in oxygen radical reactions. — J. Free. Radic. Biol. Med. 1: 3–25, 1985.PubMedCrossRefGoogle Scholar
  4. Bates, L.S., Wildren, R.P., Teary, I.D.: Rapid determination of free proline for water-stress studies. — Plant Soil 39: 205–207, 1973.CrossRefGoogle Scholar
  5. Beckman, K. B., Ames, B. N.: The free radical theory of aging matures. — Physiol. Rev. 78: 547–581, 1998.PubMedGoogle Scholar
  6. Beyer, W.F.,Jr., Fridovich, I.: Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions. — Anal. Biochem. 161: 559–566, 1987.PubMedCrossRefGoogle Scholar
  7. Campbell, D., Hurry, V., Clarke, A., Gustafsson, P., Öquist, G.: Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation. — Microbiol. Mol. Biol. Rev. 62: 667–683, 1998.PubMedGoogle Scholar
  8. Demmig-Adams, B., Adams, W.W., III, Barker, D.H., Logan, B.A., Bowling, D.R., Verhoeven, A.S.: Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation. — Physiol. Plant. 98: 253–264, 1996.CrossRefGoogle Scholar
  9. Goodman, R.N., Novacky, A.J.: The Hypersensitive Response Reaction in Plants to Pathogens, a Resistance Phenomenon. — Am. Phytopathol. Soc. Press, St. Paul 1994.Google Scholar
  10. Greenberg, J.T.: Programmed cell death: a way of life for plants. — Proc. Natl. Acad. Sci. 93: 12094–12097, 1996.PubMedCrossRefGoogle Scholar
  11. Kimura, I.: Improved purification of rice dwarf virus by use of polyethelene glycol. — Phytopathology 66: 1470–1474, 1976.CrossRefGoogle Scholar
  12. Labarca, C., Paigen, K.: A simple, rapid, and sensitive DNA assay procedure. — Anal. Biochem. 102: 344–352, 1980.PubMedCrossRefGoogle Scholar
  13. Lichtenthaler, H.K.: Chlorophylls and carotenoids — pigments of photosynthetic biomembranes. — In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol.148. Pp. 350–382. Academic Press, San Diego — New York — Berkeley — Boston — London — Sydney — Tokyo — Toronto 1987.Google Scholar
  14. Lin, X., Wang, Y.Y., Zhang, W.Z., Pu, J.X., Lin, L.S.: [Absorption spectra and fluorescence spectra of rice virus.] — Acta Biophys. Sin. 5: 304–309, 1989. [In Chin.]Google Scholar
  15. Tang, Y.L., Wen, X.G., Lu, C.M.: Differential changes in degradation of chlorophyll-protein complexes of photosystem I and photosystem II during flag leaf senescence of rice. — Plant Physiol. Biochem. 43: 193–201, 2005.PubMedCrossRefGoogle Scholar
  16. Thomas, H., Ougham, H.J., Wagstaff, C., Stead, A.D.: Defining senescence and death. — J. Exp. Bot. 54: 1127–1132, 2003.PubMedCrossRefGoogle Scholar
  17. van Doorn, W.G., Woltering, E.J.: Senescence and programmed cell death: substance or semantics. — J. Exp. Bot. 55: 2147–2153, 2004.PubMedCrossRefGoogle Scholar
  18. van Loon, L.C., Geelen, J.L.M.C.: The relation of polyphenoloxidase and peroxidase to symptom expression in tobacco var ’samsum NN’ after infection with tobacco mosaic virus. — Acta Phytopathol. Acad. Sci. Hung. 6: 9–20, 1971.Google Scholar
  19. Wang, L.F., Ji, H.B., Bai, K.Z., Li, L.B., Kuang, T.Y.: Photosystem 2 activities of hyper-accumulator Dicranopteris dichotoma Bernh from a light rare earth elements mine. — Photosynthetica 44: 202–207, 2006.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Ministry of Agriculture Key Laboratory of Rubber Biology, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Rubber Research InstituteCATASDanzhou, HainanChina

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