Light-independent accumulation of essential chlorophyll biosynthesis- and photosynthesis-related proteins in Pinus mugo and Pinus sylvestris seedlings
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Dark-grown seedlings of Pinus mugo Turra and Pinus sylvestris L. accumulate chlorophyll (Chl) and its precursor protochlorophyllide (Pchlide). Pchlide reduction is a key regulatory step in Chl biosynthesis. In the dark, Pchlide is reduced by light-independent Pchlide oxidoreductase (DPOR) encoded by three plastid genes chlL, chlN, and chlB (chlLNB). To investigate the differences in chlLNB gene expressions, we compared the dark-grown and 24-h illuminated seedlings of P. mugo and P. sylvestris. Expression of these genes was found constitutive in all analyzed samples. We report light-independent accumulation of important proteins involved in Chl biosynthesis (glutamyl-tRNA reductase) and photosystem formation (D1 and LHCI). Chl and Pchlide content and plastid ultrastructure studies were also performed.
Additional key wordschlorophyll biosynthesis conifer seedlings plastid gene expression plastid ultrastructure protochlorophyllide reduction
chlL, chlN, and chlB genes
light-independent protochlorophyllide oxidoreductase
light-dependent protochlorophyllide oxidoreductase
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This research work was supported by the grant from the Slovak Research and Development Agency (APVV-20-020805). We thank Y. Fujita and B. Grimm for kindly providing the primary antibodies used in this work and Jarmila Šramková for excellent technical assistance.
- Buchanan, B.B., Gruissem, W., Jones, R.L. (ed.): Biochemistry & Molecular Biology of Plants. 1367 pp. ASPB, Rockville 2000.Google Scholar
- Dawson, R.C.M., Elliot, D.C, Elliot, W.H., Jones, K.M.: Data for Biochemical Research. 3rd Ed. — Oxford Sci., Oxford, 1986.Google Scholar
- Dražić, G. and Mihailović, N.: Chlorophyll accumulation in black pine seedlings treated with 5-aminolevulinic acid. — Biol. Plant. 41: 277–280, 1998.Google Scholar
- Fujita, Y., Bauer, C.E.: The light-independent protochlorophyllide oxidoreductase: a nitrogenase-like enzyme catalyzing a key reaction for greening in the dark. — In: Kadish, K.M., Smith, K.M., Guilard, R. (ed.): The Porphyrine Handbook, pp.109–156. Elsevier Sci., Oxford 2003.Google Scholar
- Hudák, J., Gálová, E., Zemanová, L.: Plastid morphogenesis. — In: Pessarakli M. (ed.): Handbook of Photosynthesis. Pp. 221–245. CRC Press Taylor & Francis Group, Boca Raton — London — New York — Singapore 2005.Google Scholar
- Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. — J. Cell Biol. 27: 137, 1965.Google Scholar
- Mariani, P., De Carli, M.E., Rascio, N., Baldan, B., Casadoro, G., Gennari, G., Bodner, M., Larcher, W.: Synthesis of chlorophyll and photosynthetic competence in etiolated and greening seedlings of Larix decidua as compared with Picea abies. — J. Plant Physiol. 137: 5–14, 1990.Google Scholar
- Spano, A.J., He, Z.H., Timko, M.P.: NADPH — protochlorophyllide oxidoreductases in white pine (Pinus strobus) and loblolly pine (P. taeda) — evidence for light and developmental regulation of expression and conservation in gene organization and protein-structure between angiosperms and gymnosperms. — Mol. Gen. Genet. 236: 86–95, 1992.PubMedGoogle Scholar